Polymorphisms of the GSTT1 and GSTM1 genes in polycystic ovary syndrome
| Autor: | Mariana Mendes Porto Azevedo, Alessandra Bernadete Trovó de Marqui, Bruna Tavares Bacalá, Marly Aparecida Spadotto Balarin, Elisabete Aparecida Mantovanni Rodrigues de Resende, Marco Fábio Prata Lima, Mariana Kefalás de Oliveira Gomes, Mariangela Torreglosa Ruiz Cintra |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Reação em cadeia da polimerase multiplex
Medicine (General) Multivariate analysis Genotype Physiology 030209 endocrinology & metabolism Polimorfismo genético Logistic regression 03 medical and health sciences R5-920 0302 clinical medicine Risk Factors Medicine Humans Genetic Predisposition to Disease Family history Glutathione Transferase 030219 obstetrics & reproductive medicine Polymorphism Genetic business.industry Case-control study Síndrome do ovário policístico General Medicine Polycystic ovary genomic DNA Case-Control Studies Etiology Female business Multiplex Polymerase Chain Reaction Polycystic Ovary Syndrome |
| Zdroj: | Revista da Associação Médica Brasileira v.66 n.11 2020 Revista da Associação Médica Brasileira Associação Médica Brasileira (AMB) instacron:AMB Revista da Associação Médica Brasileira, Volume: 66, Issue: 11, Pages: 1560-1565, Published: 02 DEC 2020 Revista da Associação Médica Brasileira, Vol 66, Iss 11, Pp 1560-1565 (2020) |
| Popis: | SUMMARY BACKGROUND: This study aimed to investigate the deletion polymorphisms of the genes of the glutathione S-transferase family GSTT1 and GSTM1 in patients with Polycystic Ovarian Syndrome (PCOS), comparing them with a control population. METHODS: Blood was collected from 219 women (110 with PCOS and 109 controls) and genomic DNA was extracted. For the analysis of polymorphisms, the technique used was multiplex PCR. In the statistical analysis, the chi-square test and multiple logistic regression were used. RESULTS: There is no association between the GSTM1 null and GSTT1 null genotypes with PCOS when analyzed separately (P = 0.616 and P = 0.188). The analysis of the combined genotypes showed differences between the groups (P < 0.05), evidencing that the genotypic combination GSTT1 positive and GSTM1 negative is more frequent among patients. In the multivariate analysis, smoking was more frequent in the control group (OR = 0.22; 95% CI - 0.87-0.57; P = 0.002) while the presence of a family history of PCOS (OR = 2, 96; 95% CI - 1.54-5.68; P = 0.001) was more frequent in women with PCOS. CONCLUSIONS: In the studied sample, the deletion polymorphisms of the GSTT1 and GSTM1 genes isolated are not associated with PCOS, but in combination, they may be implicated in the etiology of the condition. RESUMO OBJETIVO: Este estudo teve como objetivo investigar os polimorfismos de deleção dos genes da família glutationa S-transferase GSTT1 e GSTM1 em pacientes com síndrome dos ovários policísticos (SOP), comparando-as com uma população controle. MÉTODOS: Foi colhido sangue de 219 mulheres (110 com SOP e 109 controles) e extraído o DNA genômico. Para análise dos polimorfismos, a técnica empregada foi PCR multiplex. Na análise estatística foi utilizado o teste do qui-quadrado e regressão logística múltipla. RESULTADOS: Não há associação dos genótipos GSTM1 nulo e GSTT1 nulo com SOP quando analisados isoladamente (p=0,616 e p=0,188). A análise dos genótipos combinados mostrou diferenças entre os grupos (p |
| Databáze: | OpenAIRE |
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