A versatile fluorescent nanobeacon lighted by DNA-templated copper nanoparticles and the application in isothermal amplification detection
| Autor: | Qingli Chai, Yuqi Wan, Yanyun Zou, Jinyang Chen, Guobin Mao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Loop-mediated isothermal amplification
Nanoparticle chemistry.chemical_element Metal Nanoparticles Sequence (biology) 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Recognition sequence Molecular beacon Instrumentation Spectroscopy Fluorescent Dyes Chemistry Thrombin DNA 021001 nanoscience & nanotechnology Fluorescence Copper Atomic and Molecular Physics and Optics 0104 chemical sciences Spectrometry Fluorescence Biophysics 0210 nano-technology |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 262 |
| ISSN: | 1873-3557 |
| Popis: | In this work, an environmentally-friendly and versatile nanobeacon was constructed by utilizing DNA-templated copper nanoparticles (CuNPs) as fluorescence signal source. As the key component of the nanobeacon, a hairpin DNA was designed to contain four segments: two segments for CuNPs template sequence, a target recognition segment and a blocking segment. At room temperature, the target recognition segment partly hybridizes with the blocking segment and thus prohibits the formation of double stranded DNA template, so that no CuNPs can be generated on the hairpin DNA. While a target is introduced, the specific binding of target with recognition sequence triggers off the conformational transformation of the hairpin DNA, which contributes to the formation of the CuNPs template. As a result, the in-situ generation of CuNPs gives birth to the fluorescence signal readout that can be used to identify the target. By reasonably varying the recognition sequence within hairpin DNA, a series of nanobeacons in response to corresponding targets, such as DNA, microRNA, thrombin, and ATP, were put forward with satisfactory sensitivity and selectivity. Moreover, this nanobeacon was also integrated with the strategy of enzyme-assisted target-recycling to realize signal amplification and ultrasensitive detection, which further demonstrated the versatility of the nanobeacon. This novel nanobeacon is expected to be a promising alternative to classical dye-labeled molecular beacon and provide new perspective on ultrasensitive fluorescence sensing. |
| Databáze: | OpenAIRE |
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