Evaluation of the performance of QuantiFERON®-TB Gold plus test in active tuberculosis patients
| Autor: | Melike Yaşar-Duman, Meltem Işıkgöz-Taşbakan, Cengiz Cavusoglu, Mehmet Sezai Taşbakan, Mehmet Nurullah Orman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Pulmonary and Respiratory Medicine medicine.medical_specialty In-Tube Test Tuberculosis QUANTIFERON-TB GOLD Infectious and parasitic diseases RC109-216 T-Cell Responses Gastroenterology Cd4 Article Assays Diseases of the respiratory system Sensitivity Internal medicine Active tb Diagnosis medicine In patient Spoligotyping RC705-779 Latent tuberculosis business.industry Signatures Active TB medicine.disease Active tuberculosis bacterial infections and mycoses LTBI Infectious Diseases QuantiFERON TB Gold Plus Infection business |
| Zdroj: | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, Vol 23, Iss, Pp 100223-(2021) |
| ISSN: | 2405-5794 |
| Popis: | The aim was to evaluate the sensitivity and the possible factors affecting the sensitivity of the QuantiFERON (R)-TB Gold Plus (QFT-Plus) assay in culture-positive active TB (Tuberculosis) patients, to investigate the possible causes of negative and indeterminate results in active TB patients, and to compare the QFT-Plus results of active TB patients and latent tuberculosis infection (LTBI) cases. The QFT-Plus assay was performed in 46 active TB patients and 64 LTBI. The sensitivity of the test was found as 79.5% in all culture-positive patients, 72.7% in the immunocompromised patients, and 86.4% in the non-immunocompromised patients. Compared to active TB, individuals with LTBI had a lower T-cell response and lower IFN-gamma concentrations. It was determined that the immunocompromisation reduced the sensitivity of the test and the secreted IFN-gamma concentrations and increased the indeterminate results in patients with active TB. There was no difference in secreted IFN-gamma concentrations between M. tuberculosis clones, but higher IFN-gamma concentrations in patients infected with M. tuberculosis strains compared to patients infected with zoonotic strains. Compared with active TB, response to only to TB2 was significantly higher in LTBI. In conclusion, it was concluded that TB2 tube increased sensitivity in LTBI but may not contribute to sensitivity in active TB. |
| Databáze: | OpenAIRE |
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