Survival of rifampin-resistant mutants of Pseudomonas fluorescens and Pseudomonas putida in soil systems
| Autor: | Boutros Jadoun Al-Achi, Stuart B. Levy, Evangelia Platsouka, G. Compeau |
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| Rok vydání: | 1988 |
| Předmět: |
Ecology
biology Strain (chemistry) Mutant Wild type Membrane Proteins Pseudomonas fluorescens Drug Resistance Microbial biology.organism_classification Applied Microbiology and Biotechnology Pseudomonas putida Microbiology Pseudomonas Pseudomonadales Mutation Rifampin Bacteria Soil Microbiology Food Science Biotechnology Pseudomonadaceae Research Article |
| Zdroj: | Applied and environmental microbiology. 54(10) |
| ISSN: | 0099-2240 |
| Popis: | The fate of spontaneous chromosomal rifampin-resistant (Rifr) mutants of Pseudomonas putida and Pseudomonas fluorescens in sterile and live organic soil from which they were isolated was studied. In sterile native-soil assays, a Rifr mutant of P. putida showed no decrease in competitive fitness when compared with the wild-type parent. However, mutants of P. fluorescens were of two general categories. Group 1 showed no difference from the wild type in terms of growth rate, competitive fitness, and membrane protein composition. Group 2 showed a slower growth rate in both minimal and enriched media and an altered membrane protein profile. These mutants also demonstrated decreased competitive fitness compared with the wild-type strain. In live soil, the Rifr P. putida strain persisted throughout the 38-day test period with a decay rate of 0.7 log10 CFU/g of soil per 10 days. A group 1 Rifr P. fluorescens mutant maintained its inoculated titer for 7 to 10 days and then decayed at a rate of 0.2 to 0.4 log10 CFU/g of soil per 10 days. A group 2 Rifr P. fluorescens mutant remained at its titer for 1 to 5 days before decaying at a two- to threefold-faster rate. These findings indicate that rifampin resistance may not be an innocuous mutation in some pseudomonads and that marked strains should be compared with wild-type parents before being used as monitors of parental strain survival. Colonization of sterile soil with either the wild-type or mutant strain precluded normal colonization of the second added strain.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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