Suppressive action of pituitary adenylate cyclase activating polypeptide (PACAP) on proliferation of immature mouse Leydig cell line TM3 cells
| Autor: | Yukio Arakawa, Masako Ohishi, Hitoshi Yanaihara, Shoji Matsumoto, Nobuo Kurokawa, Toshihiko Iwanaga |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Cell Population Receptors Pituitary Adenylate Cyclase-Activating Polypeptide Stimulation Biology General Biochemistry Genetics and Molecular Biology Cell Line Mice Internal medicine Cyclic AMP medicine Animals Testosterone Receptor education Cell Shape Cell Proliferation Mice Inbred BALB C education.field_of_study Sertoli Cells Cell growth Cell Cycle Leydig Cells General Medicine Cell cycle Sertoli cell Cell biology medicine.anatomical_structure Endocrinology Cell culture Pituitary Adenylate Cyclase-Activating Polypeptide |
| Zdroj: | Biomedical Research. 29:321-330 |
| ISSN: | 1880-313X 0388-6107 |
| DOI: | 10.2220/biomedres.29.321 |
| Popis: | We aimed in the current study to understand the participation of PACAP in stage-specific Leydig and Sertoli cell functions. For this purpose, clonal cell lines TM3 (Leydig) and TM4 (Sertoli) cells, derived from the testis of immature BALB/c mice, were used. PACAP-specific receptors were detected in TM3 cells, but not in TM4 cells, which were characterized as PAC1 (type I) receptors. Stimulation of cAMP accumulation and testosterone secretion were observed in TM3 cells during 1-2 h treatment with PACAP38 (10(-10)-10(-7) M) or PACAP27 (10(-11)-10(-7) M). After around 10 h treatment with 10(-11)-10(-7) M PACAP38 or PACAP27, proliferation of TM3 cells was suppressed in time- and dose-dependent manners, which was confirmed by real-time cell electronic sensing (RT-CES) system and phase-contrast microscopy. At 6 h after the addition of PACAP38, the percent cell population in G(2)/M phases increased significantly, while that in S phase showed significant decrease with little change in G(0)/G(1) phases. The results revealed that PACAP exerts, in addition to early stimulatory effect on cAMP formation-steroidogenesis, sustained suppressive effect on cell proliferation in TM3 cells by controlling progression of the cell cycle. The suppressive action of PACAP on proliferation in TM3 cells supports the stage-specific participation of the peptide in differentiation of immature mouse Leydig cells. |
| Databáze: | OpenAIRE |
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