Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay
| Autor: | Kazue Hirano, Chiyoji Abe, Mitsuyoshi Takahashi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Tuberculosis Asia Biology medicine.disease_cause law.invention Mycobacterium tuberculosis law medicine Humans Line Probe Assay Gene Antibiotics Antitubercular Polymerase chain reaction Mutation Drug Resistance Microbial Mycobacteriology and Aerobic Actinomycetes DNA-Directed RNA Polymerases biology.organism_classification rpoB medicine.disease Virology Molecular biology Rifampin Rifampicin medicine.drug |
| Zdroj: | Journal of clinical microbiology. 37(8) |
| ISSN: | 0095-1137 |
| Popis: | Mutations in the rpoB gene of 90 rifampin-resistant Mycobacterium tuberculosis isolates mostly from Asian countries were analyzed. Ten distinct single-nucleotide substitutions were found among the isolates by automated sequencing. A 3-nucleotide insertion was found in two isolates, and no mutation was found in five isolates (5.6%). A reverse hybridization-based line probe assay (INNO-LiPA Rif TB) for rapid detection of the mutations was evaluated with these isolates. Concordance rates with sequencing results for five wild-type probes (S probes) and four probes for specific mutations (R probes) were 96.7 and 100%, respectively. The overall concordance rate with the in vitro susceptibility testing results was 92.2% (83 of 90 isolates). These results indicate that a commercial line probe assay kit may be useful for rapid diagnosis of rifampin-resistant tuberculosis. |
| Databáze: | OpenAIRE |
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