Infliximab exerts no direct hepatotoxic effect on HepG2 cells in vitro

Autor: Henie M. J. Roelofs, Wilbert H.M. Peters, Hilbert S. de Vries, Rene H. M. te Morsche, Dirk J. de Jong, Tineke de Heij
Rok vydání: 2012
Předmět:
Hepatoblastoma
Physiology
Azathioprine
Pharmacology
Inflammatory bowel disease
6-thioguanine
6-mercaptopurine
HepG2 cells
media_common
biology
Mercaptopurine
Liver Neoplasms
Gastroenterology
Antibodies
Monoclonal
Hep G2 Cells
Antibody
Molecular gastro-enterology and hepatology Translational research [IGMD 2]
medicine.drug
Drug
musculoskeletal diseases
medicine.medical_specialty
Cell Survival
media_common.quotation_subject
macromolecular substances
In Vitro Techniques
Molecular gastro-enterology and hepatology Pathogenesis and modulation of inflammation [IGMD 2]
Sensitivity and Specificity
Internal medicine
medicine
Humans
Thioguanine
Analysis of Variance
Original Paper
Dose-Response Relationship
Drug
business.industry
Hepatotoxicity
Hepatology
Inflammatory Bowel Diseases
medicine.disease
Infliximab
digestive system diseases
In vitro
Methotrexate
Immunology
biology.protein
business
Zdroj: Digestive Diseases and Sciences, 57, 6, pp. 1604-8
Digestive Diseases and Sciences, 57, 1604-8
Digestive Diseases and Sciences
ISSN: 0163-2116
DOI: 10.1007/s10620-012-2159-7
Popis: Contains fulltext : 108348.pdf (Publisher’s version ) (Open Access) BACKGROUND: Infliximab-induced hepatotoxicity is reported in several case studies involving patients with inflammatory bowel disease (IBD) and a direct hepatotoxic effect has been proposed. OBJECTIVE: The aim of this study was to determine the direct in vitro toxicity of infliximab. As a proof of principle the in vitro toxicity of thiopurines and methotrexate was also determined. METHODS: Cell survival curves and the half maximal inhibitory concentrations (IC(50)) were obtained after 24, 48 and 72 h of incubation in HepG2 cells with the IBD drugs azathioprine, 6-mercaptopurine, 6-thioguanine, methotrexate or infliximab by using the WST-1 cytotoxicity assay. RESULTS: No in vitro hepatotoxicity in HepG2 cells was seen with infliximab, while concentration-dependent cytotoxicity was observed when HepG2 cells were incubated with increasing concentrations of azathioprine, 6-mercaptopurine and 6-thioguanine. CONCLUSION: Infliximab alone or given in combination with azathioprine showed no direct hepatotoxic effect in vitro, indicating that the postulated direct hepatotoxicity of infliximab is unlikely. 01 juni 2012
Databáze: OpenAIRE
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