Impact of dietary protein on lipid metabolism in hamsters is source-dependent and associated with changes in hepatic gene expression
| Autor: | Kevin A. Cockell, Alfred Aziz, Cristina Cruz-Hernandez, G. Sarwar Gilani, Chao Wu Xiao, W. M. Nimal Ratnayake |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Meat Glutens Gene Expression Medicine (miscellaneous) Blood lipids Biology Random Allocation chemistry.chemical_compound Cricetinae Internal medicine Casein medicine Animals RNA Messenger Cholesterol 7-alpha-Hydroxylase Receptor Phospholipids chemistry.chemical_classification Nutrition and Dietetics Mesocricetus Cholesterol Cholesterol HDL Caseins food and beverages Lipid metabolism Metabolism Lipid Metabolism Lipids Endocrinology Liver Receptors LDL chemistry Models Animal LDL receptor Fatty Acids Unsaturated Soybean Proteins Cattle lipids (amino acids peptides and proteins) Dietary Proteins Polyunsaturated fatty acid |
| Zdroj: | British Journal of Nutrition. 100:503-511 |
| ISSN: | 1475-2662 0007-1145 |
| Popis: | This study tested the hypothesis that protein source is a factor determining the impact of the diet on lipid metabolism in hamsters. Twenty-eight hamsters of similar body weight were assigned for a period of 8 weeks to one of the following four diets (seven per group) containing either 20 % (w/w) casein (CAS), beef protein (BF), wheat gluten (WG) or soya protein (SOY). The fat composition of the diet was the same (15.5 % w/w) in all groups and provided SFA, MUFA and PUFA representative of the average Canadian diet. After an overnight fast, blood and liver were collected for the measurement of serum lipids, fatty acid composition of liver phospholipids and mRNA levels of selected genes involved in lipid metabolism. WG resulted in lower total cholesterol, HDL-cholesterol and non-HDL-cholesterol but, along with SOY, in higher mRNA levels of cholesterol 7 alpha-hydroxylase and LDL receptor. Furthermore, both WG and SOY resulted in lower 18 : 3n-3, 20 : 4n-6, total n-6 PUFA, 18 : 1n-9 and total MUFA, but higher 22 : 6n-3, total n-3 PUFA, 22 : 6n-3/18 : 3n-3 and 22 : 5n-3/18 : 3n-3 ratios in liver phospholipids, and higher hepatic Delta6-desaturase mRNA levels. These results show that the impact of dietary protein on lipid metabolism is source-dependent and associated with changes in mRNA abundances of key hepatic enzymes and receptors. |
| Databáze: | OpenAIRE |
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