Neuronal branching of sensory neurons is associated with BDNF‐positive eosinophils in atopic dermatitis
| Autor: | Manuela Gehring, Nika Kotnik, Nikolaos Patsinakidis, Daria Guseva, Urda Rüdrich, Konstantin Agelopoulos, Ulrike Raap, Evgeni Ponimaskin, Alexander Kapp, Bernhard Homey, Sonja Ständer, Bernhard F. Gibbs |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Adolescent Sensory Receptor Cells Neurite Immunology Dermatitis Atopic 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neurotrophic factors medicine Humans Immunology and Allergy Axon biology Brain-Derived Neurotrophic Factor Dermis Eosinophil Eosinophils 030104 developmental biology medicine.anatomical_structure nervous system 030228 respiratory system chemistry biology.protein Female Epidermis Free nerve ending Histamine Sensory nerve Neurotrophin |
| Zdroj: | Clinical & Experimental Allergy. 50:577-584 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/cea.13560 |
| Popis: | Background Pruritus is a major symptom of atopic dermatitis (AD) and is transmitted by a subpopulation of non-myelinated C-type free nerve endings in the epidermis and upper dermis. Stimulation of these nerve terminals is affected by histamine, neurotrophins and physical factors. Eosinophils of patients with AD are a source of neurotrophins, including brain-derived neurotrophic factor (BDNF), levels of which correlate with disease severity. Objective The purpose of this study was to determine the anatomical localization of eosinophils in the skin of patients with AD with regard to peripheral nerves and to investigate whether eosinophils induce sprouting and neurite outgrowth in murine sensory neurons. Methods Cryosections of skin derived from AD and control (NA) patients were subjected to immunofluorescence analysis with markers for eosinophils, BDNF and neuronal cells. Stimulated eosinophil supernatants were used for the treatment of cultured peripheral mouse dorsal root ganglia (DRG) neurons followed by morphometric analysis. Results Dermal axon density and the proximity of eosinophils to nerve fibres were significantly higher in AD patients vs NA. Both neuronal projections and eosinophils expressed BDNF. Furthermore, activated eosinophil supernatants induced BDNF-dependent mouse DRG neuron branching. Conclusions and clinical relevance Our results indicate that BDNF-positive eosinophils are also localized in close proximity with nerve fibres in AD, suggesting a functional relationship between BDNF-expressing eosinophils and neuronal projections. These observations suggest that eosinophils may have considerable impact on pruritus by supporting sensory nerve branching. |
| Databáze: | OpenAIRE |
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