Genetic diversity of Pantoea stewartii subspecies stewartii causing jackfruit-bronzing disease in Malaysia

Autor: Rohaya Ibrahim, Ganesan Vadamalai, Daljit Singh Karam, Nuraizat Abidin, Siti Izera Ismail, Noor Wahida Ismail-Suhaimy, Mansor Hakiman, Mohd Termizi Yusof, Dzarifah Zulperi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Artificial Gene Amplification and Extension
Pathogenesis
Plant Science
Subspecies
Pathology and Laboratory Medicine
01 natural sciences
Polymerase Chain Reaction
Geographical Locations
Database and Informatics Methods
Medicine and Health Sciences
Flowering Plants
Phylogeny
Data Management
Genetics
Multidisciplinary
biology
Phylogenetic tree
Virulence
Eukaryota
Phylogenetic Analysis
Plants
Housekeeping gene
Bacterial Typing Techniques
Phylogenetics
Medicine
DNA
Intergenic
Sequence Analysis
Research Article
Nicotiana
DNA
Bacterial
Computer and Information Sciences
Asia
Bioinformatics
Science
Sequence Databases
Research and Analysis Methods
Fruits
03 medical and health sciences
Genetic variation
Evolutionary Systematics
Molecular Biology Techniques
Molecular Biology
Taxonomy
Plant Diseases
Genetic diversity
Evolutionary Biology
Pantoea
Organisms
Malaysia
Biology and Life Sciences
Genetic Variation
Plant Pathology
biology.organism_classification
rpoB
030104 developmental biology
Biological Databases
Genes
Bacterial
People and Places
Multilocus sequence typing
Artocarpus
010606 plant biology & botany
Multilocus Sequence Typing
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 6, p e0234350 (2020)
ISSN: 1932-6203
Popis: Jackfruit-bronzing is caused by bacteria Pantoea stewartii subspecies stewartii (P. stewartii subsp. stewartii), showing symptoms of yellowish-orange to reddish discolouration and rusty specks on pulps and rags of jackfruit. Twenty-eight pure bacterial strains were collected from four different jackfruit outbreak collection areas in Peninsular Malaysia (Jenderam, Maran, Muadzam Shah and Ipoh). Positive P. stewartii subsp. stewartii verification obtained in the study was based on the phenotypic, hypersensitivity, pathogenicity and molecular tests. Multilocus sequence analysis (MLSA) was performed using four housekeeping genes (gyrB, rpoB, atpD and infB) on all 28 bacterial strains. Single gyrB, rpoB, atpD and infB phylogenetic trees analyses revealed the bootstrap value of 99-100% between our bacterial strains with P. stewartii subsp. stewartii reference strains and P. stewartii subsp. indologenes reference strains. On the other hand, phylogenetic tree of the concatenated sequences of the four housekeeping genes revealed that our 28 bacterial strains were more closely related to P. stewartii subsp. stewartii (99% similarities) compared to its close relative P. stewartii subsp. indologenes, although sequence similarity between these two subspecies were up to 100%. All the strains collected from the four collection areas clustered together, pointing to no variation among the bacterial strains. This study improves our understanding and provided new insight on the genetic diversity of P. stewartii subsp. stewartii associated with jackfruit-bronzing in Malaysia.
Databáze: OpenAIRE
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