Flt3 Ligand Regulates the Development of Innate Lymphoid Cells in Fetal and Adult Mice
| Autor: | Edit Horvath, Antonius G. Rolink, David Voehringer, Matthias Kreuzaler, Daniela Finke, Selina Sitte, Nicole von Burg, Annick Peter, Anne Baerenwaldt |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adoptive cell transfer Cellular differentiation Immunology Mice Transgenic Cell Separation Biology 03 medical and health sciences Mice Peyer's Patches 0302 clinical medicine Fetus medicine Immunology and Allergy Animals Lymphopoiesis Progenitor cell skin and connective tissue diseases Mice Knockout Innate lymphoid cell Membrane Proteins Cell Differentiation Lymphoid Progenitor Cells Flow Cytometry Adoptive Transfer Immunohistochemistry Immunity Innate Cell biology Mice Inbred C57BL body regions 030104 developmental biology medicine.anatomical_structure Lymphatic system Bone marrow 030215 immunology |
| Zdroj: | J Immunol |
| DOI: | 10.4049/jimmunol.1501380 |
| Popis: | Flt3 ligand (Flt3L) promotes survival of lymphoid progenitors in the bone marrow and differentiation of dendritic cells (DCs), but its role in regulating innate lymphoid cells (ILCs) during fetal and adult life is not understood. By using Flt3L knockout and transgenic mice, we demonstrate that Flt3L controls ILC numbers by regulating the pool of α4β7− and α4β7+ lymphoid tissue inducer cell progenitors in the fetal liver and common lymphoid progenitors in the bone marrow. Deletion of flt3l severely reduced the number of fetal liver progenitors and lymphoid tissue inducer cells in the neonatal intestine, resulting in impaired development of Peyer’s patches. In the adult intestine, NK cells and group 2 and 3 ILCs were severely reduced. This effect occurred independently of DCs as ILC numbers were normal in mice in which DCs were constitutively deleted. Finally, we could show that administration of Flt3L increased the number of NKp46− group 3 ILCs in wild-type and even in Il7−/− mice, which generally have reduced numbers of ILCs. Taken together, Flt3L significantly contributes to ILC and Peyer’s patches development by targeting lymphoid progenitor cells during fetal and adult life. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|