Selective adsorption of L1210 leukemia cells/human leukocytes on micropatterned surfaces prepared from polystyrene/polypropylene-polyethylene blends

Autor: Ayse B. Tekinay, Nevin Atalay Gengec, H. Yildirim Erbil, Hilal Unal Gulsuner
Přispěvatelé: Unal, Hilal Unal, Tekinay, Ayse Begum
Rok vydání: 2014
Předmět:
polyethylene
Polymers
wettability
Diseases
Selective adsorption
polyethylene polypropylene copolymer
surface free energy
Contact angle
chemistry.chemical_compound
Colloid and Surface Chemistry
Selective cell adhesion
Leukocytes
Micropatterning
Micropatterned surface
contact angle
Cells
Cultured
chemistry.chemical_classification
Leukemia
article
Surfaces and Interfaces
General Medicine
Polymer
Phase-separation process
unclassified drug
polyvinyl alcohol
priority journal
Polyethylene
polymerization
microscopy
Micro patterning
Wetting
leukocyte
Biotechnology
ethylene vinyl acetate copolymer
Materials science
surface property
leukemia L 1210
Surface Properties
Cells
Commercial polymers
polystyrene
cell selection
Polypropylenes
Dip-coating
Adsorption
L1210 leukemia cells
Cell Line
Tumor
Polymer chemistry
Cell Adhesion
Humans
human
Physical and Theoretical Chemistry
cell viability
Adsorption performance
copolymer
human cell
Blending
Surface energy
chemistry
Chemical engineering
adsorption
Polystyrenes
Polystyrene
Leukemia cells
cell structure
Zdroj: Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765
Popis: The objective of this study is to prepare polymeric surfaces which will adsorb L1210 leukemia cells selectively more than that of healthy human leukocytes in order to develop new treatment options for people with leukemia. Chemically heterogeneous and micropatterned surfaces were formed on round glass slides by dip coating with accompanying phase-separation process where only commercial polymers were used. Surface properties were determined by using optical microscopy, 3D profilometry, SEM and measuring contact angles. Polymer, solvent/nonsolvent types, blend composition and temperature were found to be effective in controlling the dimensions of surface microislands. MTT tests were applied for cell viability performance of these surfaces. Polystyrene/polyethylene-polypropylene blend surfaces were found to show considerable positive selectivity to L1210 leukemia cells where L1210/healthy leukocytes adsorption ratio approached to 9-fold in vitro. Effects of wettability, surface free energy, microisland size geometry on the adsorption performances of L1210/leukocytes pairs are discussed. © 2013 Elsevier B.V.
Databáze: OpenAIRE
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