Selective adsorption of L1210 leukemia cells/human leukocytes on micropatterned surfaces prepared from polystyrene/polypropylene-polyethylene blends
Autor: | Ayse B. Tekinay, Nevin Atalay Gengec, H. Yildirim Erbil, Hilal Unal Gulsuner |
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Přispěvatelé: | Unal, Hilal Unal, Tekinay, Ayse Begum |
Rok vydání: | 2014 |
Předmět: |
polyethylene
Polymers wettability Diseases Selective adsorption polyethylene polypropylene copolymer surface free energy Contact angle chemistry.chemical_compound Colloid and Surface Chemistry Selective cell adhesion Leukocytes Micropatterning Micropatterned surface contact angle Cells Cultured chemistry.chemical_classification Leukemia article Surfaces and Interfaces General Medicine Polymer Phase-separation process unclassified drug polyvinyl alcohol priority journal Polyethylene polymerization microscopy Micro patterning Wetting leukocyte Biotechnology ethylene vinyl acetate copolymer Materials science surface property leukemia L 1210 Surface Properties Cells Commercial polymers polystyrene cell selection Polypropylenes Dip-coating Adsorption L1210 leukemia cells Cell Line Tumor Polymer chemistry Cell Adhesion Humans human Physical and Theoretical Chemistry cell viability Adsorption performance copolymer human cell Blending Surface energy chemistry Chemical engineering adsorption Polystyrenes Polystyrene Leukemia cells cell structure |
Zdroj: | Colloids and Surfaces B: Biointerfaces |
ISSN: | 0927-7765 |
Popis: | The objective of this study is to prepare polymeric surfaces which will adsorb L1210 leukemia cells selectively more than that of healthy human leukocytes in order to develop new treatment options for people with leukemia. Chemically heterogeneous and micropatterned surfaces were formed on round glass slides by dip coating with accompanying phase-separation process where only commercial polymers were used. Surface properties were determined by using optical microscopy, 3D profilometry, SEM and measuring contact angles. Polymer, solvent/nonsolvent types, blend composition and temperature were found to be effective in controlling the dimensions of surface microislands. MTT tests were applied for cell viability performance of these surfaces. Polystyrene/polyethylene-polypropylene blend surfaces were found to show considerable positive selectivity to L1210 leukemia cells where L1210/healthy leukocytes adsorption ratio approached to 9-fold in vitro. Effects of wettability, surface free energy, microisland size geometry on the adsorption performances of L1210/leukocytes pairs are discussed. © 2013 Elsevier B.V. |
Databáze: | OpenAIRE |
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