Characterization of OsMIK in a rice mutant with reduced phytate content reveals an insertion of a rearranged retrotransposon
| Autor: | Qingyao Shu, Hairui Cui, Haijun Zhao, Yves Poirier, Junjie Fu, Guo-Zhen Liu, Xiu-Hong Xu, Yuanyuan Tan |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Plant Phytic Acid Retroelements Blotting Western Mutant Retrotransposon Locus (genetics) Biology Real-Time Polymerase Chain Reaction Chromosomes Plant Exon Gene Expression Regulation Plant Genetics RNA Messenger Gene Plant Proteins Gene Rearrangement Reverse Transcriptase Polymerase Chain Reaction Intron Chromosome Mapping food and beverages Oryza Promoter General Medicine DNA Methylation Molecular biology Blotting Southern Mutagenesis Insertional Phenotype Mutation lipids (amino acids peptides and proteins) biological phenomena cell phenomena and immunity Homologous recombination Agronomy and Crop Science Biotechnology |
| Zdroj: | Theoretical and Applied Genetics. 126:3009-3020 |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/s00122-013-2189-3 |
| Popis: | The rice low phytic acid (lpa) mutant Os-lpa-XS110-1(XS-lpa) has ~45 % reduction in seed phytic acid (PA) compared with the wild-type cultivar Xiushui 110. Previously, a single recessive gene mutation was shown to be responsible for the lpa phenotype and was mapped to a region of chromosome 3 near OsMIK (LOC_Os03g52760) and OsIPK1 (LOC_Os03g51610), two genes involved in PA biosynthesis. Here, we report the identification of a large insert in the intron of OsMIK in the XS-lpa mutant. Sequencing of fragments amplified through TAIL-PCRs revealed that the insert was a derivative of the LINE retrotransposon gene LOC_Os03g56910. Further analyses revealed the following characteristics of the insert and its impacts: (1) the inserted sequence of LOC_Os03g56910 was split at its third exon and rejoined inversely, with its 5' and 3' flanking sequences inward and the split third exon segments outward; (2) the LOC_Os03g56910 remained in its original locus in XS-lpa, and the insertion probably resulted from homologous recombination repair of a DNA double strand break; (3) while the OsMIK transcripts of XS-lpa and Xiushui 110 were identical, substantial reductions of the transcript abundance (~87 %) and the protein level (~60 %) were observed in XS-lpa, probably due to increased methylation in its promoter region. The above findings are discussed in the context of plant mutagenesis, epigenetics and lpa breeding. |
| Databáze: | OpenAIRE |
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