Characterization of glucuronic acid conjugates of a novel angiotensin receptor antagonist
| Autor: | Soraya Y. Hughes, Gary R. Manchee, D. E. Games, Peter J Eddershaw, Janet Oxford, Gary D. Bowers |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Tetrazoles Thermospray Glucuronates Mass spectrometry High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Matrix (chemical analysis) Angiotensin Receptor Antagonists chemistry.chemical_compound Dogs Chromatography detector Animals Bile Chromatography High Pressure Liquid Spectroscopy Chromatography Chemistry Organic Chemistry Nicotinic Acids Glucuronic acid Rats Biochemistry Injections Intravenous Indicators and Reagents Spectrophotometry Ultraviolet Drug metabolism Conjugate |
| Zdroj: | Rapid Communications in Mass Spectrometry. 8:217-221 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.1290080218 |
| Popis: | Nanogram quantities of glucuronic acid conjugates of GR117289 in rat and dog bile have been analysed by semi-microbore high-performance liquid chromatography (HPLC)/ionspray mass spectrometry with on-line UV diode array detection. The determination of drug metabolites in bile has often proved problematical due to the large number of endogenous components in this biological matrix, in particular the bile acids. Semi-microbore HPLC is useful for concentrating small quantities of material and, in combination with an on-line diode array detector, for distinguishing between drug related and endogenous components. A novel angiotensin II receptor antagonist, GR117289, had proved difficult to analyse by thermospray mass spectrometry because of its thermal lability. The use of the less thermally dependent technique of ionspray mass spectrometry allowed the characterization of nanogram quantities of glucuronic acid metabolites of GR117289 in bile. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text