Aldehyde dehydrogenase 3I1 gene is recruited in conferring multiple abiotic stress tolerance in plants
| Autor: | S A Zafar, D Bartles, H H Kirch, M R Khan, H Raza |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Transgene
Plant Science Genetically modified crops medicine.disease_cause chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Arabidopsis Botany medicine Ecology Evolution Behavior and Systematics Plant Proteins Abiotic component Aldehydes biology Abiotic stress fungi food and beverages General Medicine Aldehyde Dehydrogenase Malondialdehyde biology.organism_classification Plants Genetically Modified Droughts Metabolic pathway chemistry Oxidative stress |
| Zdroj: | Plant biology (Stuttgart, Germany)REFERENCES. 24(1) |
| ISSN: | 1438-8677 |
| Popis: | Plant growth and productivity is restricted by a multitude of abiotic stresses. These stresses negatively affect physiological and metabolic pathways, leading to the production of many harmful substances like ROS, lipid peroxides and aldehydes. This study was conducted to investigate the role of Arabidopsis ALDH3I1 gene in multiple abiotic stress tolerance. Transgenic tobacco plants were generated that overexpress the ALDH3I1 gene driven by the CaMV35S promoter and evaluated under different abiotic stresses, namely salt, drought, cold and oxidative stress. Tolerance to stress was evaluated based on responses of various growth and physiological traits under stress condition. Transgenic plants displayed elevated ALDH3I1 transcript levels compared to WT plants. The constitutive ectopic expression of ALDH3I1 conferred increased tolerance to salt, drought, cold and oxidative stresses in transgenic plants, along with improved plant growth. Transgenic plants overexpressing ALDH3I1 had higher chlorophyll content, photosynthesis rate and proline, and less accumulation of ROS and malondialdehyde compared to the WT, which contributed to stress tolerance in transgenic plants. Our results further revealed that ALDH3I1 had a positive effect on CO2 assimilation rate in plants under abiotic stress conditions. Overall, this study revealed that ALDH3I1 positively regulates abiotic stress tolerance in plants, and has future implications in producing transgenic cereal and horticultural plants tolerant to abiotic stresses. |
| Databáze: | OpenAIRE |
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