Studies on the platelet radioactive anti-immunoglobulin test
| Autor: | I. Mahn, Cornelius Dienst, Karl-Heinz Sauer, Christian Mueller-Eckhardt, Gregor Schulz |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Blood Platelets
Cell number Immunology Standard procedure Iodine Radioisotopes Antibody Specificity HLA Antigens Methods medicine Animals Humans Immunology and Allergy Platelet Hla antibodies Incubation Sensitization Chemistry Immune Sera Complement Fixation Tests Complement fixation test Antibodies Anti-Idiotypic medicine.anatomical_structure Immunoglobulin G Immunoglobulin test Rabbits |
| Zdroj: | Journal of Immunological Methods. 19:1-11 |
| ISSN: | 0022-1759 |
| Popis: | A platelet radioactive antiimmunoglobulin test (PRAT) has recently been introduced. In the present report, a detailed analysis of the influence of varying test conditions (i.e. effeciency of iodination of anti-IgG, cell number, degree of sensitization of platelets, times and temperatures of incubation, frequency of washings) on the results of this technique is presented. A standard procedure based on these findings is outlined. It is shown that the PRAT is two to four times more sensitive than platelet complement fixation for the detection of HLA antibodies. |
| Databáze: | OpenAIRE |
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