Argon Inhalation for 24 Hours After Onset of Permanent Focal Cerebral Ischemia in Rats Provides Neuroprotection and Improves Neurologic Outcome
| Autor: | Wei Yang, Dennis A. Turner, Dongmei Chu, Huaxin Sheng, Ulrike Hoffmann, Jennifer A. Creed, Litao Li, Yu-Mi Ryang, David S. Warner, Shuang Ma |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Ischemia Critical Care and Intensive Care Medicine Neuroprotection Article Brain Ischemia Brain ischemia 03 medical and health sciences Random Allocation 0302 clinical medicine Text mining In vivo medicine Animals Argon Rats Wistar Stroke Random allocation Inhalation business.industry 030208 emergency & critical care medicine medicine.disease Rats Disease Models Animal Neuroprotective Agents 030228 respiratory system Anesthesia business |
| Zdroj: | Crit Care Med |
| ISSN: | 1530-0293 |
| Popis: | OBJECTIVES: We tested the hypothesis that prolonged inhalation of 70% argon for 24 hours after in-vivo permanent or temporary stroke provides neuroprotection, and improves neurologic outcome and overall recovery after 7 days. DESIGN: Controlled, randomized, double-blinded laboratory study SETTING: Animal research laboratories SUBJECTS: Adult Wistar male rats (n=110) INTERVENTIONS: Rats were subjected to permanent or temporary focal cerebral ischemia via middle cerebral artery occlusion, followed by inhalation of 70% argon or nitrogen in 30% oxygen for 24 hours. On postoperative day 7, a 48-point neuroscore and histologic lesion size were assessed. MEASUREMENTS AND MAIN RESULTS: After argon inhalation for 24 hours immediately following severe permanent ischemia induction, neurologic outcome (neuroscore, p=0.034), overall recovery (body weight, p=0.02), and infarct volume (total infarct volume p=0.0001, cortical infarct volume p=0.0003, subcortical infarct volume p=0.0001) were significantly improved. When 24-hour argon treatment was delayed for 2 hours after permanent stroke induction, or until after post-ischemic reperfusion treatment, neurologic outcomes remained significantly improved (neuroscore, p=0.043 and p=0.014, respectively), as was overall recovery (body weight, p=0.015), compared to nitrogen treatment. However, infarct volume and 7-day mortality were not significantly reduced when argon treatment was delayed. CONCLUSIONS: Neurologic outcome (neuroscore), overall recovery (body weight), and infarct volumes were significantly improved after 24-hour inhalation of 70% argon administered immediately after severe permanent stroke induction. Neurologic outcome and overall recovery were also significantly improved even when argon treatment was delayed for 2 hours or until after reperfusion. |
| Databáze: | OpenAIRE |
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