Rat Chondrocyte-Associated Antigen Identified as Sialylated Transmembrane Protein Tmp21 Belonging to the p24 Protein Family
| Autor: | Anna Osiecka-Iwan, Stanislaw Moskalewski, Justyna Niderla-Bielińska, Anna Hyc |
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| Jazyk: | angličtina |
| Předmět: |
Cartilage
Articular Chondrocyte-associated antigen Endocrinology Diabetes and Metabolism Biology Tmp21 Chondrocyte p24 protein symbols.namesake Chondrocytes Endocrinology Western blot Antigen medicine Animals Orthopedics and Sports Medicine Aggrecans Collagen Type II Cells Cultured Aggrecan Original Research medicine.diagnostic_test Endoplasmic reticulum Membrane Proteins Cell Differentiation Golgi apparatus Molecular biology Transmembrane protein Rats Transplantation medicine.anatomical_structure Animals Newborn Rats Inbred Lew symbols Rat Rabbits |
| Zdroj: | Calcified Tissue International |
| ISSN: | 0171-967X |
| DOI: | 10.1007/s00223-013-9816-5 |
| Popis: | Rabbit serum produced after transplantation of isolated rat chondrocytes [sensitized rabbit serum (SRS)] demonstrated M r ~ 74- and ~23-kDa (western blot analysis) antigens in rat chondrocyte extracts. Only the latter remained after reduction in 2-mercaptoethanol. Protein sequence analysis of 23-kDa chondrocyte-associated antigen (CAA) revealed that it corresponds to transmembrane Tmp21 protein belonging to the p24 protein family. These proteins mainly participate in the traffic between the endoplasmic reticulum and Golgi complex and in some cells appear also in the membrane of secretory granules and plasmalemma. Tmp21 extracted from chondrocytes was sialylated and ceased to bind SRS after deglycosylation. A previous study from our laboratory indicated that expression of CAA, now identified as sialylated Tmp21, decreased in cultured chondrocytes concomitantly with the decline of collagen type II and aggrecan and the rise of collagen type I and versican expression. Since the sialylated form of Tmp21 (also known as emp24) was not described in other tissues and seems to be specific for chondrocytes, we assume that CAA may be considered a chondrocyte differentiation antigen. |
| Databáze: | OpenAIRE |
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