Cell Wall Teichoic Acid Glycosylation in Listeria monocytogenes Serotype 4b Requires gtcA , a Novel, Serogroup-Specific Gene
| Autor: | Nattawan Promadej, Shaynoor Dramsi, S. Kathariou, Franz Fiedler, Pascale Cossart |
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| Přispěvatelé: | University of Hawai'i [Honolulu] (UH), Ludwig-Maximilians-Universität München (LMU), Institut Pasteur [Paris], This research was partially supported by U.S. Department of Agriculture Competitive Research Initiative AAFS grant 92-37 201-8095, by ILSI—North America, and by a seed grant from the University of Hawaii., Institut Pasteur [Paris] (IP) |
| Rok vydání: | 1999 |
| Předmět: |
Serotype
Glycosylation MESH: Sequence Homology Amino Acid Operon [SDV]Life Sciences [q-bio] Restriction Mapping Mutant MESH: Amino Acid Sequence MESH: Listeria monocytogenes medicine.disease_cause MESH: Antibodies Monoclonal chemistry.chemical_compound Cell Wall MESH: Reverse Transcriptase Polymerase Chain Reaction Carbohydrate Conformation Cloning Molecular MESH: Carbohydrate Conformation MESH: Bacterial Proteins MESH: Restriction Mapping 0303 health sciences Teichoic acid Reverse Transcriptase Polymerase Chain Reaction Antibodies Monoclonal MESH: Glycosylation Complementation Carbohydrate Sequence Lipoteichoic acid MESH: Genes Bacterial Physiology and Metabolism Molecular Sequence Data MESH: Sequence Alignment Biology Microbiology Open Reading Frames 03 medical and health sciences MESH: Cell Wall Bacterial Proteins Listeria monocytogenes medicine MESH: Cloning Molecular Amino Acid Sequence Serotyping Molecular Biology 030304 developmental biology MESH: Carbohydrate Sequence MESH: Molecular Sequence Data Sequence Homology Amino Acid 030306 microbiology MESH: Serotyping MESH: Open Reading Frames Molecular biology Teichoic Acids chemistry Genes Bacterial MESH: Teichoic Acids Galactose Sequence Alignment |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 1999, 181 (2), pp.418-425. ⟨10.1128/JB.181.2.418-425.1999⟩ Journal of Bacteriology, 1999, 181 (2), pp.418-425. ⟨10.1128/JB.181.2.418-425.1999⟩ |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.181.2.418-425.1999 |
| Popis: | We have identified a novel gene, gtcA , involved in the decoration of cell wall teichoic acid of Listeria monocytogenes serotype 4b with galactose and glucose. Insertional inactivation of gtcA brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. Complementation of the mutants with the cloned gtcA in trans restored galactose and glucose on teichoic acid to wild-type levels. The complemented strains also recovered reactivity with c74.22. Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. In serotype 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology to Bacillus subtilis rpmE , encoding ribosomal protein L31. In contrast to gtcA , the latter gene appears conserved among all screened serotypes of L. monocytogenes . |
| Databáze: | OpenAIRE |
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