Hydrophilic Interaction Liquid Chromatography-Electrospray Ionization Mass Spectrometry for Therapeutic Drug Monitoring of Metformin and Rosuvastatin in Human Plasma
| Autor: | George Migias, Ariadni Vonaparti, Giorgos Machairas, Irene Panderi, Dimitra Gennimata, Vasiliki Brakoulia, Constantinos Pistos, Nikolaos Antonopoulos |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Electrospray
Spectrometry Mass Electrospray Ionization Electrospray ionization therapeutic drug monitoring Pharmaceutical Science Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Liquid chromatography–mass spectrometry Drug Discovery Ammonium formate Protein precipitation Humans Sample preparation Physical and Theoretical Chemistry Rosuvastatin Calcium HILIC Chromatography Hydrophilic interaction chromatography 010401 analytical chemistry Organic Chemistry nutritional and metabolic diseases 3. Good health 0104 chemical sciences LC-MS chemistry Chemistry (miscellaneous) Molecular Medicine metformin rosuvastatin Chromatography Liquid |
| Zdroj: | Molecules Volume 23 Issue 7 Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules, Vol 23, Iss 7, p 1548 (2018) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules23071548 |
| Popis: | In this work a hydrophilic interaction liquid chromatography/positive ion electrospray mass spectrometric assay (HILIC/ESI-MS) has been developed and fully validated for the quantitation of metformin and rosuvastatin in human plasma. Sample preparation involved the use of 100 &mu L of human plasma, following protein precipitation and filtration. Metformin, rosuvastatin and 4-[2-(propylamino) ethyl] indoline 2 one hydrochloride (internal standard) were separated by using an X-Bridge-HILIC BEH analytical column (150.0 × 2.1 mm i.d., particle size 3.5 &mu m) with isocratic elution. A mobile phase consisting of 12% (v/v) 15 mM ammonium formate water solution in acetonitrile was used for the separation and pumped at a flow rate of 0.25 mL min&minus 1. The linear range of the assay was 100 to 5000 ng mL&minus 1 and 2 to 100 ng mL&minus 1 for metformin and rosuvastatin, respectively. The current HILIC-ESI/MS method allows for the accurate and precise quantitation of metformin and rosuvastatin in human plasma with a simple sample preparation and a short a chromatographic run time (less than 15 min). Plasma samples from eight patients were further analysed proving the capability of the proposed method to support a wide range of clinical studies. |
| Databáze: | OpenAIRE |
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