Esculetin exerts anti-proliferative effects against non-small-cell lung carcinoma by suppressing specificity protein 1 in vitro
| Autor: | Il-Keun Kong, Minseok Kim, Ko Sungho, Jung-Hyun Shim, Jae-Cheon Shin, Young Joo Jeon, Ra Ham Lee, Jeong-Yun Jang, Seon-Min Park, Jung-Il Chae, Seokho Kim, Hak-Jae Chung, Keon Bong Oh, Jae-Min Seo, Jin H Cho |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lung Neoplasms Physiology Sp1 Transcription Factor Cell Biophysics Regulator Down-Regulation Antineoplastic Agents Antioxidants 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Survivin Carcinoma medicine Umbelliferones Transcription factor Cell Proliferation Dose-Response Relationship Drug Chemistry General Medicine Cell cycle medicine.disease In vitro 030104 developmental biology medicine.anatomical_structure Apoptosis 030220 oncology & carcinogenesis Cancer research |
| Zdroj: | General physiology and biophysics. 36(1) |
| ISSN: | 0231-5882 |
| Popis: | Esculetin, a coumarin derivative, is a phenolic compound isolated from Artemisia capillaris, Citrus limonia, and Euphorbia lathyris. Although it has been reported to have anti-inflammatory, anti-oxidant, and anti-proliferative activities in several human cancers, its anti-proliferative activity against non-small-cell lung carcinoma (NSCLC) and the molecular mechanisms involved have not been adequately elucidated. In this study, we used two NSCLC cell lines (NCI-H358 and NCI-H1299) to investigate the anti-proliferative activity and apoptotic effect of esculetin. Our data showed that esculetin-treated cells exhibited reduced proliferation and apoptotic cell morphologies. Intriguingly, the transcription factor specificity protein 1 (Sp1) was significantly suppressed by esculetin in a dose- and time-dependent manner. Furthermore, the levels of p27 and p21, two key regulators of the cell cycle, were up-regulated by the esculetin-mediated down-regulation of Sp1; the level of a third cell-cycle regulator, survivin, was decreased, resulting in caspase-dependent apoptosis. Therefore, we conclude that esculetin could be a potent anti-proliferative agent in patients with NSCLC. |
| Databáze: | OpenAIRE |
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