High prevalence of international ESBL CTX-M-15-producingEnterobacter cloacaeST114 clone in animals
| Autor: | Jean Yves Madec, Estelle Saras, Cécile Ponsin, Marisa Haenni, Marie Petitjean, Didier Hocquet, Safia Dahmen |
|---|---|
| Přispěvatelé: | Unité Antibiorésistance et Virulence Bactériennes, Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail ( ANSES Maisons-Alfort ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Genotype 030106 microbiology beta-Lactamases Microbiology law.invention 03 medical and health sciences Dogs Plasmid Disk Diffusion Antimicrobial Tests [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology law Enterobacter cloacae Prevalence Pulsed-field gel electrophoresis Animals Pharmacology (medical) Typing Polymerase chain reaction Pharmacology Molecular Epidemiology biology Molecular epidemiology Enterobacteriaceae Infections Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification Electrophoresis Gel Pulsed-Field Molecular Typing Blotting Southern [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Infectious Diseases Cats Multilocus sequence typing France Multilocus Sequence Typing Plasmids |
| Zdroj: | Journal of Antimicrobial Chemotherapy Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2016 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkw006 |
| Popis: | OBJECTIVES The objective of this study was to characterize ESBL-producing Enterobacter cloacae isolated from animals and to compare their clonal distribution with that of human-related isolates. METHODS Among 635 clinical E. cloacae from horses, dogs and cats collected in France between 2010 and 2013, 36 were resistant to ceftiofur as determined by disc diffusion. ESBL genes were identified by sequencing. Plasmids carrying ESBL-encoding genes were characterized by PCR-based replicon typing, S1-PFGE and Southern blotting. IncHI2 plasmids were subtyped using the plasmid double-locus sequence typing scheme and multiplex amplification of the hipA, smr0092 and smr0183 genes. All E. cloacae were typed by PFGE and MLST. ST clustering was analysed by eBURST. RESULTS All 36 ceftiofur-resistant E. cloacae produced an ESBL. Their PFGE patterns formed 23 clusters of high similarity and 13 STs and were isolated from epidemiologically unrelated animals (14 horses, 11 dogs and 11 cats) distributed throughout France. ST114, the most prevalent clone in humans, was over-represented in animals (16/36) compared with other human-related clones detected here. The blaCTX-M-15 gene was dominant (66.7%) and mostly carried on IncHI2 plasmids (ST1 subtype). ST114 isolates always produced CTX-M-15. CONCLUSIONS Most ESBL-producing E. cloacae from animals studied here (69.4%) belonged to potentially high-risk clones in humans, in particular ST114 (44.4%). These data raise questions and potential concerns about the transfer of E. cloacae between animals and humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|