Protective Effects of Sulphonated Formononetin in a Rat Model of Cerebral Ischemia and Reperfusion Injury

Autor: Fei Lin, Jian Hou, Li-bo Zou, Jingwei Tian, Jie He, Haibo Zhu
Rok vydání: 2014
Předmět:
Male
Vascular Endothelial Growth Factor A
Angiogenesis
Neovascularization
Physiologic
Pharmaceutical Science
Apoptosis
Pharmacology
Umbilical vein
Brain Ischemia
Analytical Chemistry
Rats
Sprague-Dawley
chemistry.chemical_compound
Drug Discovery
Human Umbilical Vein Endothelial Cells
Animals
Humans
Medicine
Tube formation
Platelet Endothelial Cell Adhesion Molecule
Plant Extracts
business.industry
Organic Chemistry
Brain
Endothelial Cells
Infarction
Middle Cerebral Artery
Astragalus Plant
medicine.disease
Isoflavones
Rats
Platelet Endothelial Cell Adhesion Molecule-1
Vascular endothelial growth factor
Vascular endothelial growth factor B
Disease Models
Animal
Vascular endothelial growth factor A
Neuroprotective Agents
Complementary and alternative medicine
chemistry
Reperfusion Injury
Injections
Intravenous
Immunology
Molecular Medicine
Angiogenesis Inducing Agents
business
Reperfusion injury
Phytotherapy
Zdroj: Planta Medica. 80:262-268
ISSN: 1439-0221
0032-0943
DOI: 10.1055/s-0033-1360340
Popis: Sodium formononetin-3′-sulphonate is a derivative of the plant isoflavone formononetin. The present study aimed to investigate the neuroprotective and angiogenesis effects of sodium formononetin-3′-sulphonate in vivo and in vitro. Treatment with sodium formononetin-3′-sulphonate (3, 7.5, 15, and 30 mg/kg, intravenous injection) could protect the brain from ischemia and reperfusion injury by improving neurological function, suppressing cell apoptosis, and increasing expression levels of vascular endothelial growth factor and platelet endothelial cell adhesion molecule 1 by middle cerebral artery occlusion. Treatment with sodium formononetin-3′-sulphonate (10 and 20 µg/mL) significantly increased cell migration, tube formation, and vascular endothelial growth factor and platelet endothelial cell adhesion molecule levels in human umbilical vein endothelial cells. Our results suggest that sodium formononetin-3′-sulphonate provides significant neuroprotective effects against cerebral ischemia and reperfusion injury in rats, and improves cerebrovascular angiogenesis in human umbilical vein endothelial cells. The protective mechanisms of sodium formononetin-3′-sulphonate may be attributed to the suppression of cell apoptosis and improved cerebrovascular angiogenesis by promoting vascular endothelial growth factor and platelet endothelial cell adhesion molecule expression.
Databáze: OpenAIRE
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