Active immunization against the proregions of GDF9 or BMP15 alters ovulation rate and litter size in mice
| Autor: | Steve Lawrence, Jennifer L. Juengel, C Joy McIntosh, Peter R. Smith, Kenneth P. McNatty |
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| Rok vydání: | 2011 |
| Předmět: |
Litter (animal)
Male Ovulation endocrine system Embryology medicine.medical_specialty Litter Size media_common.quotation_subject Growth Differentiation Factor 9 Ovary Growth differentiation factor-9 Biology Active immunization Mice Endocrinology Pregnancy Internal medicine medicine Animals Humans Ovulation Inhibition Vaccines Contraceptive Protein Precursors media_common Mice Inbred BALB C Bone morphogenetic protein 15 Vaccination Obstetrics and Gynecology Cell Biology Oocyte Protein Structure Tertiary medicine.anatomical_structure HEK293 Cells Reproductive Medicine biology.protein Female Bone Morphogenetic Protein 15 Keyhole limpet hemocyanin |
| Zdroj: | Reproduction (Cambridge, England). 143(2) |
| ISSN: | 1741-7899 |
| Popis: | The transforming growth factor β (TGFB) superfamily proteins bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), are essential for mammalian fertility. Recent in vitro evidence suggests that the proregions of mouse BMP15 and GDF9 interact with their mature proteins after secretion. In this study, we have actively immunized mice against these proregions to test the potential in vivo roles on fertility. Mice were immunized with either N- or C-terminus proregion peptides of BMP15 or GDF9, or a full-length GDF9 proregion protein, each conjugated to keyhole limpet hemocyanin (KLH). For each immunization group, ovaries were collected from ten mice for histology after immunization, while a further 20 mice were allowed to breed and litter sizes were counted. To link the ovulation and fertility data of these two experimental end points, mice were joined during the time period identified by histology as being the ovulatory period resulting in to the corpora lutea (CL) counted. Antibody titers in sera increased throughout the study period, with no cross-reactivity observed between BMP15 and GDF9 sera and antigens. Compared with KLH controls, mice immunized with the N-terminus BMP15 proregion peptide had ovaries with fewer CL (PPPP |
| Databáze: | OpenAIRE |
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