Dehydrothermally Cross-Linked Collagen Membrane with a Bone Graft Improves Bone Regeneration in a Rat Calvarial Defect Model
| Autor: | Young Ku Heo, Jung Seok Lee, Yin Zhe An, Seong-Ho Choi, Ui Won Jung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
collagen medicine.medical_specialty allograft endocrine system Materials science Rat model bone regeneration cross-linking dehydrothermal Calvaria lcsh:Technology Article 03 medical and health sciences 0302 clinical medicine Internal medicine medicine General Materials Science Bone formation Bone regeneration lcsh:Microscopy lcsh:QC120-168.85 Calvarial defect lcsh:QH201-278.5 lcsh:T Collagen membrane Biomaterial 030206 dentistry 030104 developmental biology medicine.anatomical_structure Endocrinology lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) Bone volume lcsh:TK1-9971 hormones hormone substitutes and hormone antagonists |
| Zdroj: | Materials; Volume 10; Issue 8; Pages: 927 Materials, Vol 10, Iss 8, p 927 (2017) Materials |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma10080927 |
| Popis: | In this study, the bone regeneration efficacy of dehydrothermally (DHT) cross-linked collagen membrane with or without a bone graft (BG) material was evaluated in a critical-sized rat model. An 8-mm-diameter defect was created in the calvaria of 40 rats, which were randomized into four groups: (1) control; (2) DHT; (3) BG; and, (4) DHT + BG. Evaluations were made at 2 and 8 weeks after surgery using micro-computed tomographic (micro-CT), histological, and histomorphometric analyses. Micro-CT analysis showed an increase in the new bone volume (NBV) of the BG and DHT + BG groups at 2 weeks after surgery, representing a significant difference (p < 0.05). At 8 weeks after surgery, the NBV increased in all four groups. However, larger NBVs were observed in the BG and DHT + BG groups, and a significant difference was no longer observed between the two groups. Histologic analysis demonstrated that the graft materials sustained the center of the defect in the BG and DHT + BG groups, which was shown in histomorphometric analysis as well. These results suggest that DHT membrane is a safe biomaterial with adequate tissue integration, and has a positive effect on new bone formation. Moreover, the best effects were achieved when DHT was used in conjunction with BG materials. |
| Databáze: | OpenAIRE |
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