Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet
| Autor: | Alexander Krüttgen, Christian Cornelissen, Mathias W. Hornef, Michael Dreher, Matthias Imöhl, Michael Kleines |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology serology Antibodies Viral Sensitivity and Specificity Article Serology COVID-19 Serological Testing 03 medical and health sciences Virology Humans skin and connective tissue diseases Immunoassay biology SARS-CoV-2 fungi COVID-19 respiratory tract diseases body regions 030104 developmental biology Immunoglobulin G biology.protein ELISA Antibody |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 1879-0984 |
| Popis: | Highlights • The sensitivity and specificity of seven serological assays for SARS-CoV-2 were compared • No single assay offered a combination of very high sensitivity and very high specificity • To maximize sensitivity and specificity two assays should be combined There is an ongoing need for highly reliable serological assays to detect individuals with past SARS-CoV-2 infection. Using 75 sera from patients tested positive or negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the Liaison SARS-CoV-2 S1/S2 IgG assay (DiaSorin), the Elecsys Anti-SARS-CoV-2 assay (Roche), and the ID Screen SARS-CoV-2-N IgG indirect kit (IDVet). We determined a sensitivity of 95.5%, 95.5%, and 100% and a specificity of 90.5%, 96.2%, and 92,5% for the DiaSorin assay, the Roche assay, and the IDVet assay, respectively. We conclude that serologic assays combining very high sensitivity and specificity are still not commercially available for SARS-CoV-2. For maximizing sensitivity and specificity of SARS-CoV-2 serological diagnostics, the combination of two assays may be helpful. |
| Databáze: | OpenAIRE |
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