Super-Resolution Ultrasound Localization Microscopy on a Rabbit Liver VX2 Tumor Model: An Initial Feasibility Study
| Autor: | Wei Zhang, Rita J. Miller, Matthew R. Lowerison, Pengfei Song, Zhijie Dong, Krista A. Keller |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Liver tumor Acoustics and Ultrasonics Computer science Microscopy Acoustic Biophysics 01 natural sciences Article 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine In vivo 0103 physical sciences Microscopy medicine Animals Vx2 tumor Radiology Nuclear Medicine and imaging 010301 acoustics Radiological and Ultrasound Technology business.industry Liver Neoplasms Ultrasound medicine.disease Superresolution Subpixel rendering Disease Models Animal Microbubbles Feasibility Studies Rabbits business Biomedical engineering |
| Zdroj: | Ultrasound Med Biol |
| ISSN: | 0301-5629 |
| DOI: | 10.1016/j.ultrasmedbio.2021.04.012 |
| Popis: | Ultrasound localization microscopy can image microvasculature in vivo without sacrificing imaging penetration depth. However, the reliance on super-resolution inference limits the applicability of the technique as subpixel tissue motion can corrupt microvascular reconstruction. Consequently, the majority of previous pre-clinical research on this super-resolution procedure has been restricted to low motion experimental models with ample motion correction and/or data rejection, which precludes the imaging of organ sites with that exhibit a high degree of respiratory and other motion. In this paper, we present a novel anesthesia protocol in rabbits that induces safe controllable periods of apnea to enable the long imaging acquisition times required for ultrasound localization microscopy. We apply this protocol to a VX2 liver tumor model undergoing sorafenib therapy and compare the results to super-resolution images from a conventional high-dose isoflurane anesthesia. We found that the apneic protocol was necessary to correctly identify the poorly vascularized tumor cores, as verified by immunohistochemistry, and to reveal the tumoral microvascular architecture. |
| Databáze: | OpenAIRE |
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