Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
| Autor: | Tewodros Debebe, Jürgen König, Angelika Pointner, Alexander G. Haslberger, Ingrid Höfinger, Stephanie Lilja, Berit Hippe, Kalina Duszka, Carina Stoll, Ulrike Krammer |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty food.ingredient Pyruvate dehydrogenase kinase SIRT3 PDK4 Gut flora Article Gene Expression Regulation Enzymologic Catalysis lcsh:Chemistry Inorganic Chemistry food Internal medicine Lactobacillus Ketogenesis medicine Humans Sirtuins Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Aged Buchinger fasting Clostridiales gut microbiota biology Christensenella Organic Chemistry Fasting General Medicine Middle Aged biology.organism_classification Gastrointestinal Microbiome Computer Science Applications sirtuin Endocrinology lcsh:Biology (General) lcsh:QD1-999 Sirtuin biology.protein Female |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 2331, p 2331 (2021) International Journal of Molecular Sciences Volume 22 Issue 5 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22052331 |
| Popis: | Periodic fasting (PF) is an increasingly popular approach that assists in the management of metabolic and inflammatory diseases as well as in preventing mechanisms involved in aging. However, little is known about the effects of fasting on gut microbiota and its impact on the epigenetic regulation of metabolically relevant enzymes, especially sirtuins (SIRTs). We analyzed the effect of periodic fasting on the human gut microbiota, SIRTs expression, and mitochondrial content in 51 males and females. The participants fasted under supervision for five consecutive days following the Buchinger fasting guidelines. Ketogenesis, selected mRNAs, miRNAs, mitochondrial (mt) DNA, and gut composition were analyzed before and after PF. PF triggered a significant switch in metabolism, as indicated by the increase in ß-hydroxybutyrate (BHB) and pyruvate dehydrogenase kinase isoform 4 (PDK4) expression in the capillary blood. MtDNA, SIRT1, SIRT3, and miRlet7b-5p expression in blood cells were elevated, whereas SIRT6 and miR125b-5p were not affected. Following fasting, gut microbiota diversity increased, and a statistically significant correlation between SIRT1 gene expression and the abundance of Prevotella and Lactobacillus was detected. The abundance of longevity related Christensenella species increased after fasting and inversely correlated with age as well as body mass index (BMI). Thus, this represents the first study that showing that fasting not only changes the composition of the gut microbiota, making it more diverse, but also affects SIRT expression in humans. |
| Databáze: | OpenAIRE |
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