Müller glial cells induce stem cell properties in retinal progenitors in vitro and promote their further differentiation into photoreceptors
| Autor: | Beatriz de los Santos, Maria V. Simon, Carolina E. Abrahan, Luis E. Politi, Pablo De Genaro, Nora P. Rotstein |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Opsin
Neurogenesis MULLER CELLS Biology Retina Ciencias Biológicas Cellular and Molecular Neuroscience Neural Stem Cells medicine Animals Photoreceptor Cells Rats Wistar Progenitor cell RETINA Cells Cultured PHOTORECEPTORS Stem Cells Regeneration (biology) Biología del Desarrollo Cell Differentiation Peripherin Cell cycle Coculture Techniques eye diseases Rats Neuroepithelial cell medicine.anatomical_structure Animals Newborn sense organs Stem cell Neuroglia Neuroscience CIENCIAS NATURALES Y EXACTAS STEM CELLS |
| DOI: | 10.1002/jnr.22747/abstract |
| Popis: | Using stem cells to replace lost neurons is a promising strategy for treating retinal neurodegenerative diseases. Among their multiple functions, Müller glial cells are retina stem cells, with a robust regenerative potential in lower vertebrates, which is much more restricted in mammals. In rodents, most retina progenitors exit the cell cycle immediately after birth, differentiate as neurons, and then cannot reenter the cell cycle. Here we demonstrate that, in mixed cultures with Müller glial cells, rat retina progenitor cells expressed stem cell properties, maintained their proliferative potential, and were able to preserve these properties and remain mitotically active after several consecutive passages. Notably, these progenitors retained the capacity to differentiate as photoreceptors, even after successive reseedings. Müller glial cells markedly stimulated differentiation of retina progenitors; these cells initially expressed Crx and then developed as mature photoreceptors that expressed characteristic markers, such as opsin and peripherin. Moreover, they were light responsive, insofar as they decreased their cGMP levels when exposed to light, and they also showed high-affinity glutamate uptake, a characteristic of mature photoreceptors. Our present findings indicate that, in addition to giving rise to new photoreceptors, Muller glial cells might instruct a pool of undifferentiated cells to develop and preserve stem cell characteristics, even after successive reseedings, and then stimulate their differentiation as functional photoreceptors. This complementary mechanism might contribute to enlarge the limited regenerative capacity of mammalian Müller cells. Fil: Simon, Maria Victoria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: de Genaro, Pablo Adrian. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: de Los Santos, Elisa Beatriz. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina |
| Databáze: | OpenAIRE |
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