Cloning and sequencing of mRNAs coding for two adult α globin chains of the salamander Pleurodeles waltlii
| Autor: | Jean Rosa, Anne-Marie Duprat, Colette Valentin, Paul-Henri Romeo, Michelle Flavin, Michel Cohen-Solal |
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| Rok vydání: | 1986 |
| Předmět: |
Amphibian
Macromolecular Substances Xenopus Molecular cloning Pleurodeles biology.animal Complementary DNA Genetics Animals Coding region Amino Acid Sequence RNA Messenger Globin Cloning Molecular Base Sequence biology cDNA library Protein primary structure DNA DNA Restriction Enzymes General Medicine Salamandridae biology.organism_classification Molecular biology Globins Genes |
| Zdroj: | Gene. 42:159-168 |
| ISSN: | 0378-1119 |
| Popis: | A cDNA library of erythrocyte mRNAs was established from immature red blood cells of the adult amphibian, Pleurodeles waltlii (urodele; salamander). The cDNA clones corresponding to the four adult globin chains were first identified and characterized by positive selection and the cDNAs derived from the two (major and minor) α-globin chains sequenced. The sequences presented contain both the complete 3'-noncoding region and the coding region of both chains, with the exception of the first nine codons of the minor α-chain, and a portion of the 5'-noncoding region of the major chain. The amino acid sequences of the encoded α-globin polypeptides have been deduced and compared with those of Xenopus laevis and of man. These comparative studies suggest that the α-globins of Pleurodeles waltlii and Xenopus laevis may have diverged from a common ancestral gene at the time when mammalian and amphibian lines diverged, and that they then evolved separately. Duplication of the α-gene, which is responsible for the polypeptide heterogeneity, appears to have occurred earlier in Pleurodeles waltlii than in Xenopus laevis . |
| Databáze: | OpenAIRE |
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