Microbial community compositions in breast implant biofilms associated with contracted capsules

Autor: Paul A. Kenward, Rachel L. Simister, Aaron C Van Slyke, Jenifer S. Spence, Nick J. Carr, Sean A. Crowe, Peter A. Lennox
Rok vydání: 2021
Předmět:
0301 basic medicine
Medical Implants
Artificial Gene Amplification and Extension
030230 surgery
Biochemistry
Polymerase Chain Reaction
law.invention
0302 clinical medicine
law
Medicine and Health Sciences
DNA extraction
Breast augmentation
Multidisciplinary
Microbiota
Genomics
Capsular contracture
Nucleic acids
Actinobacteria
Ribosomal RNA
Medical Microbiology
Breast implant
Engineering and Technology
Medicine
medicine.symptom
Research Article
Biotechnology
Cell biology
Cellular structures and organelles
Breast Implants
Science
Capsules
Bioengineering
Microbial Genomics
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Extraction techniques
Implant Capsular Contracture
Genetics
medicine
Humans
Microbiome
Non-coding RNA
Molecular Biology Techniques
Molecular Biology
Biology and life sciences
Bacteria
Organisms
Biofilm
Capsule
Bacteriology
030104 developmental biology
Metagenomics
Biofilms
RNA
Medical Devices and Equipment
Contracture
Bacterial Biofilms
Ribosomes
Zdroj: PLoS ONE, Vol 16, Iss 4, p e0249261 (2021)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0249261
Popis: Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determine capsule biofilm microbial community compositions, we employed amplicon sequencing of the 16S rRNA gene using DNA extracted from breast implant capsule samples. These cultivation independent analyses revealed that capsule associated biofilms are more diverse than canonical single-species infections, but have relatively low diversity (~
Databáze: OpenAIRE