Whole egg, but not egg white, ingestion induces mTOR colocalization with the lysosome after resistance exercise
Autor: | Sidney Abou Sawan, Stephan van Vliet, Daniel W. D. West, Joseph W. Beals, Nicholas A. Burd, Daniel R. Moore, Scott A. Paluska |
---|---|
Rok vydání: | 2018 |
Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Physiology Eggs Muscle Proteins Biology Immunofluorescence Eating Mice Young Adult 03 medical and health sciences 0302 clinical medicine Lysosomal-Associated Membrane Protein 2 Internal medicine Lysosome medicine Animals Humans Ingestion RNA Messenger Muscle Skeletal Exercise PI3K/AKT/mTOR pathway Rapid Report medicine.diagnostic_test TOR Serine-Threonine Kinases Egg Proteins Colocalization Resistance Training Cell Biology 030104 developmental biology Endocrinology medicine.anatomical_structure Protein Biosynthesis biology.protein Dietary Proteins TSC2 Lysosomes 030217 neurology & neurosurgery RHEB Egg white |
Zdroj: | American Journal of Physiology-Cell Physiology. 315:C537-C543 |
ISSN: | 1522-1563 0363-6143 |
Popis: | We have recently demonstrated that whole egg ingestion induces a greater muscle protein synthetic (MPS) response when compared with isonitrogenous egg white ingestion after resistance exercise in young men. Our aim was to determine whether whole egg or egg white ingestion differentially influenced colocalization of key regulators of mechanistic target of rapamycin complex 1 (mTORC1) as means to explain our previously observed divergent postexercise MPS response. In crossover trials, 10 healthy resistance-trained men (21 ± 1 yr; 88 ± 3 kg; body fat: 16 ± 1%; means ± SE) completed lower body resistance exercise before ingesting whole eggs (18 g protein, 17 g fat) or egg whites (18 g protein, 0 g fat). Muscle biopsies were obtained before exercise and at 120 and 300 min after egg ingestion to assess, by immunofluorescence, protein colocalization of key anabolic signaling molecules. After resistance exercise, tuberous sclerosis 2-Ras homolog enriched in brain (Rheb) colocalization decreased ( P < 0.01) at 120 and 300 min after whole egg and egg white ingestion with concomitant increases ( P < 0.01) in mTOR-Rheb colocalization. After resistance exercise, mTOR-lysosome-associated membrane protein 2 (LAMP2) colocalization significantly increased at 120 and 300 min only after whole egg ingestion ( P < 0.01), and mTOR-LAMP2 colocalization correlated with rates of MPS at rest and after exercise ( r = 0.40, P < 0.05). We demonstrated that the greater postexercise MPS response with whole egg ingestion is related in part to an enhanced recruitment of mTORC1-Rheb complexes to the lysosome during recovery. These data suggest nonprotein dietary factors influence the postexercise regulation of mRNA translation in human skeletal muscle. |
Databáze: | OpenAIRE |
Externí odkaz: |