Suckling stimulus suppresses messenger RNA for tyrosine hydroxylase in arcuate neurons during lactation
| Autor: | Wei-Wei Le, Thomas G. Sherman, Gloria E. Hoffman, Kathie A. Berghorn |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Cytoplasm Hypothalamo-Hypophyseal System Time Factors Transcription Genetic Tyrosine 3-Monooxygenase Down-Regulation In situ hybridization Biology Gene Expression Regulation Enzymologic Rats Sprague-Dawley Catecholamines Dopamine Internal medicine Lactation Gene expression medicine Animals RNA Messenger In Situ Hybridization Cell Nucleus Messenger RNA Tyrosine hydroxylase General Neuroscience Arcuate Nucleus of Hypothalamus Immunohistochemistry Prolactin Animals Suckling Rats Up-Regulation Endocrinology medicine.anatomical_structure Hypothalamus Sucking Behavior Female medicine.drug |
| Zdroj: | The Journal of comparative neurology. 438(4) |
| ISSN: | 0021-9967 |
| Popis: | Tyrosine hydroxylase (TH) mRNA in tuberoinfundibular dopamine (TIDA) neurons is suppressed during lactation but rebounds upon pup removal. A time course of TH mRNA changes after pup removal revealed three phases: (1) a nuclear phase (evident 1.5 hours after pup removal, maximal at 3 hours) with TH mRNA appearing in 1 or 2 nuclear loci with little or no change in cytoplasmic mRNA; (2) a cytoplasmic phase (noted 6 hours after pup removal, peaked 12‐24 hours) with a significant increase in total TH mRNA levels mainly in the cytoplasm; and (3) a stabilization phase (24 ‐ 48 hours after pup removal) when nuclear signals were low and cytoplasmic RNA showed a slight decline with extension of RNA clusters into the cell dendrites. In rats whose pups could suckle only on one side, TH was up-regulated only on the side contralateral to nipple blockade. These data indicate that after suckling terminates, TH up-regulation is evident at 1.5 hours, but 6 hours is needed before the cells transport sufficient mRNA into the cytoplasm. The rapid signaling of TH upregulation stems from the fact that the TIDA neurons respond to neural signals from termination of suckling. J. Comp. Neurol. 438:423‐ 432, 2001. © 2001 Wiley-Liss, Inc. Indexing terms: in situ hybridization; nuclear transcription sites; hypothalamus; dopamine; neuroendocrine; prolactin; gene expression |
| Databáze: | OpenAIRE |
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