Folic acid is necessary for proliferation and differentiation of C2C12 myoblasts
| Autor: | Seong Yeon Hwang, Bokyung Sung, Hong Jae Kim, Cheol Min Kim, Nam Deuk Kim, Jin-Hong Shin, Hye Jin Oh, Na Lam Hwang, Mi-Ae Yoo, Yu Ra Ahn, Yong Jung Kang, Jung Yoon Jang, Hae Young Chung |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Time Factors Physiology Myoblasts Skeletal Clinical Biochemistry Folic Acid Deficiency Biology Muscle Development Cell Line Mice 03 medical and health sciences Folic Acid Internal medicine medicine Animals Myocyte Cellular Senescence Myogenin Cell Proliferation Myosin Heavy Chains Myogenesis Cell Cycle food and beverages Skeletal muscle Cell Differentiation Cell Biology Metabolism Cell cycle musculoskeletal system B vitamins 030104 developmental biology Endocrinology medicine.anatomical_structure Biochemistry C2C12 DNA Damage |
| Zdroj: | Journal of Cellular Physiology. 233:736-747 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.25989 |
| Popis: | Folic acid, a water soluble B vitamin, plays an important role in cellular metabolic activities, such as functioning as a cofactor in one-carbon metabolism for DNA and RNA synthesis as well as nucleotide and amino acid biosynthesis in the body. A lack of dietary folic acid can lead to folic acid deficiency and result in several health problems, including macrocytic anemia, elevated plasma homocysteine, cardiovascular disease, birth defects, carcinogenesis, muscle weakness, and walking difficulty. However, the effect of folic acid deficiency on skeletal muscle development and its molecular mechanisms are unknown. We, therefore, investigated the effect of folic acid deficiency on myogenesis in skeletal muscle cells and found that folic acid deficiency induced proliferation inhibition and cell cycle breaking as well as cellular senescence in C2C12 myoblasts, implying that folic acid deficiency influences skeletal muscle development. Folic acid deficiency also inhibited differentiation of C2C12 myoblasts and induced deregulation of the cell cycle exit and many cell cycle regulatory genes. It inhibited expression of muscle-specific marker MyHC as well as myogenic regulatory factor (myogenin). Moreover, immunocytochemistry and Western blot analyses revealed that DNA damage was more increased in folic acid-deficient medium-treated differentiating C2C12 cells. Furthermore, we found that folic acid resupplementation reverses the effect on the cell cycle and senescence in folic acid-deficient C2C12 myoblasts but does not reverse the differentiation of C2C12 cells. Altogether, the study results suggest that folic acid is necessary for normal development of skeletal muscle cells. |
| Databáze: | OpenAIRE |
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