Association of Nitric Oxide Synthase2 gene polymorphisms with leprosy reactions in northern Indian population
| Autor: | Harish Sagar, Keshar Kunja Mohanty, J K Chakma, Amit Dubey, Sameer S. Bhagyawant, Raj Kamal, Sanjay Kumar Biswas, Ekata Sinha, Mohan Natarajan, Mamta Arora |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Linkage disequilibrium 030231 tropical medicine Population Gene Expression India Nitric Oxide Synthase Type II Single-nucleotide polymorphism Polymorphism Single Nucleotide Microbiology Linkage Disequilibrium 03 medical and health sciences 0302 clinical medicine Gene Frequency Leprosy Genotype Genetics medicine Humans Allele Promoter Regions Genetic education Molecular Biology Mycobacterium leprae Alleles Ecology Evolution Behavior and Systematics education.field_of_study Models Genetic biology Haplotype Middle Aged biology.organism_classification medicine.disease 030104 developmental biology Infectious Diseases Haplotypes Case-Control Studies Immunology Female |
| Zdroj: | Infection, Genetics and Evolution. 51:67-73 |
| ISSN: | 1567-1348 |
| DOI: | 10.1016/j.meegid.2017.03.015 |
| Popis: | The pathogen Mycobacterium leprae causes leprosy that affects mainly skin and nerves. Polymorphisms of certain genes are substantiated to be associated with the susceptibility/resistance to leprosy. The present investigation addressed the association of Nitric Oxide Synthase2 gene polymorphisms and leprosy in a population from northern part of India. A total of 323 leprosy cases and 288 healthy controls were genotyped for four NOS2 promoter variants (rs1800482, rs2779249, rs8078340 and rs2301369) using FRET technology in Real Time PCR. None of these SNPs in promoter sites was associated with susceptibility/resistance to leprosy. NOS2 rs1800482 was found to be monomorphic with GG genotype. However, NOS2-1026T allele was observed to be in higher frequency with leprosy cases (BL and LL) who were not suffering from any reactional episodes compared to cases with ENL reaction {OR=0.30, 95% CI (0.10-0.86), p=0.024}. NOS2-1026GT genotype was more prevalent in cases without reaction (BT, BB and BL) compared to RR reactional patients {OR=0.38, 95% CI (0.17-0.86), p=0.02}. Although haplotype analysis revealed that no haplotype was associated with leprosy susceptibility/resistance with statistical significance, GTG haplotype was noted to be more frequent in healthy controls. These SNPs are observed to be in linkage disequilibrium. Although, these SNPs are not likely to influence leprosy vulnerability, -1026G>T SNP was indicated to have noteworthy role in leprosy reactions. |
| Databáze: | OpenAIRE |
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