American tegumentary leishmaniasis: T-cell differentiation profile of cutaneous and mucosal forms—co-infection with Trypanosoma cruzi

Autor: Alejandra Barrio, Ana G. González Prieto, Cecilia Parodi, Patricia Baré, María Celia Mora, Maria M.E. de Bracco, María F. García Bustos, Federico Ramos, Miguel A. Basombrío
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
CD4-Positive T-Lymphocytes
Leishmaniasis
Mucocutaneous
Male
Cellular differentiation
Ciencias de la Salud
CD8-Positive T-Lymphocytes
0302 clinical medicine
Immunophenotyping
T-Lymphocyte Subsets
Immunology and Allergy
Peripheral T Lymphocytes
Parasitología
Child
Aged
80 and over
Coinfection
CD28
Cell Differentiation
General Medicine
Middle Aged
Female
Microbiology (medical)
Adult
CIENCIAS MÉDICAS Y DE LA SALUD
Adolescent
030231 tropical medicine
Immunology
Biology
03 medical and health sciences
Interferon-gamma
Young Adult
Cutaneous leishmaniasis
Antigens
CD
medicine
Humans
Chagas Disease
Interleukin-7 receptor
Aged
Perforin
Cutaneous Leishmaniasis
Differentiation And Memory Phenotype
medicine.disease
Leishmania
biology.organism_classification
Mucosal Leishmaniasis
030104 developmental biology
T cell differentiation
Leishmania&Ndash;T. Cruzi Co-Infection
CD8
Follow-Up Studies
Popis: American tegumentary leishmaniasis displays two main clinical forms: cutaneous (CL) and mucosal (ML). ML is more resistant to treatment and displays a more severe and longer evolution. Since both forms are caused by the same Leishmania species, the immunological response of the host may be an important factor determining the evolution of the disease. Herein, we analyzed the differentiation and memory profile of peripheral CD4+ and CD8+ T lymphocytes of patients with CL and ML and their Leishmania–T. cruzi co-infected counterparts. We measured the expression of CD27, CD28, CD45RO, CD127, PD-1 and CD57, together with interferon-γ and perforin. A highly differentiated phenotype was reflected on both T subsets in ML and preferentially on CD8+ T cells in CL. A positive trend toward a higher T differentiation profile was found in T. cruzi-infected CL and ML patients as compared with Leishmania single infections. Association between CD8+ T-cell differentiation and illness duration was found within the first year of infection, with progressive increase of highly differentiated markers over time. Follow-up of patients with good response to therapy showed predominance of early differentiated CD8+ T cells and decrease of highly differentiated cells, while patients with frequent relapses presented the opposite pattern. CD8+ T cells showed the most striking changes in their phenotype during leishmaniasis. Patients with long-term infections showed the highest differentiated degree implying a relation between T differentiation and parasite persistence. Distinct patterns of CD8+ T differentiation during follow-up of different clinical outcomes suggest the usefulness of this analysis in the characterization of Leishmania-infected patients. Fil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Garcia Bustos, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Barrio, Alejandra. Universidad Nacional de Salta. Facultad de Ciencias de la Salud; Argentina Fil: Ramos, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: González Prieto, Ana Gabriela. Universidad Nacional de Salta. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mora, Maria Celia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Basombrío, Miguel Ángel Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: de Elizalde, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Databáze: OpenAIRE
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