IL-10 expression by CT26 colon carcinoma cells inhibits their malignant phenotype and induces a T cell-mediated tumor rejection in the context of a systemic Th2 response
| Autor: | S Klein, Alicia I. Bravo, Cecilia Carbone, Yuti Chernajovsky, Maria A. Jasnis, M. F. Ledda, Osvaldo L. Podhajcer, Eduardo Chuluyan, Soraya Adris |
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| Rok vydání: | 1999 |
| Předmět: |
Bioquímica
CD4-Positive T-Lymphocytes Male medicine.medical_treatment T cell Genetic Vectors Biology CD8-Positive T-Lymphocytes Interferon-gamma Mice Immune system Th2 Cells Tumor immunity Genetics medicine Tumor Cells Cultured Animals Humans Neoplasm Invasiveness Molecular Biology Gene transfer Interleukin 4 Analysis of Variance Mice Inbred BALB C Gene Transfer Techniques Immunotherapy Genetic Therapy Flow Cytometry Interleukin-10 Interleukin 10 Cytokine medicine.anatomical_structure Retroviridae Cell culture Immunoglobulin G Immunology Colonic Neoplasms Cancer research Molecular Medicine Cytokines Interleukin-4 CD8 Spleen |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 0969-7128 |
| Popis: | In spite of the evidence that IL-10 has Th1-immunosuppressive and anti-inflammatory effects, it has been shown that IL-10 may reduce the tumorigenic capacity of certain tumor cell types. In order to characterize the responses elicited by IL-10, we explored the effect of transducing murine CT26 colon carcinoma cells with a recombinant retrovirus expressing mIL-10. IL-10 gene transfer of CT26 cells had no effect on tumor cell growth on plastic surface but inhibited the anchorage-independent growth capacity of tumor cells and their metastatic potential as assessed by their invasive and migration ability. Expression of IL-10 also elicited an antitumor immune response involving both CD4+ and CD8+ T cells. Assessment of the immune status of the animals demonstrated that mice injected with CT26-IL10 cells showed prevalence of a systemic and tumor-specific Th2 response. Spleen cells obtained from these mice showed an increased production of IL-4 and no changes in IFNgamma levels, characteristic of a Th2 response. These results demonstrate that IL-10 affects CT26 tumor cell growth by both inhibiting the malignant phenotype and by recruiting and activating a T cell-mediated antitumor response. This T cell response occurs in the context of a shift towards a Th2 response. Facultad de Ciencias Veterinarias |
| Databáze: | OpenAIRE |
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