Sensitivity and Specificity of Multiphoton Laser Tomography for In Vivo and Ex Vivo Diagnosis of Malignant Melanoma
| Autor: | Enrico Dimitrow, Johannes Norgauer, Martin Kaatz, Karsten König, Peter Elsner, Martin Johannes Koehler, Mirjana Ziemer |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Skin Neoplasms Adolescent Biopsy Dermatology Sensitivity and Specificity Biochemistry law.invention Young Adult law In vivo medicine Humans Nevus Child Melanoma Tomography Molecular Biology Aged Aged 80 and over Observer Variation Nevus Pigmented medicine.diagnostic_test business.industry Lasers Reproducibility of Results Cancer Dendritic Cells Cell Biology Middle Aged medicine.disease Laser Logistic Models Microscopy Fluorescence Multiphoton Child Preschool Melanocytes Female business Ex vivo |
| Zdroj: | Journal of Investigative Dermatology. 129:1752-1758 |
| ISSN: | 0022-202X |
| DOI: | 10.1038/jid.2008.439 |
| Popis: | The incidence of malignant melanoma has shown a dramatic increase over the past three decades. Patient outcome and curability depend on early diagnosis. In vivo multiphoton laser tomography represents a recently developed diagnostic tool that allows non-invasive tissue imaging. We aim to demonstrate the application of multiphoton laser tomography for the in vivo differentiation and diagnosis of melanoma. Laser radiation in the near infrared spectrum was used to image endogenous fluorophores by multiphoton excitation. Eighty-three melanocytic skin lesions have been investigated. The results showed distinct morphological differences in melanoma compared with melanocytic nevi. In particular, six characteristic features of malignant melanoma were specified and statistically evaluated. Sensitivity values up to 95% (range: 71–95%) and specificity values up to 97% (range: 69–97%) were achieved for diagnostic classification. Logistic regression analysis was performed to identify the most significant diagnostic criteria. We found that architectural disarray of the epidermis, poorly defined keratinocyte cell borders as well as the presence of pleomorphic or dendritic cells were of prime importance. By means of this procedure accuracy values up to 97% were reached. These findings underline the potential applicability of multiphoton laser tomography in melanoma diagnosis of melanocytic skin lesions. |
| Databáze: | OpenAIRE |
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