A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us?
| Autor: | Jorge Fraga Nodarse, Raúl Cordoví Prado, Lissette Pérez Santos, Rigoberto Fimia Duarte, Lucy J. Robertson, Marina del Carmen Sánchez Romero, Luis Enrique Jerez Puebla, Fidel Ángel Núñez Fernández, I Rodríguez, Sahily de la Caridad Ortega Medina, Iraís Atencio Millán, Ubaldo del Risco |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Veterinary medicine Mitochondrial DNA Epidemiology Infectious and parasitic diseases RC109-216 Molecular confirmation Taenia solium parasitic diseases Diagnosis medicine Parasite hosting Taeniosis biology Taenia Incidence (epidemiology) Cuba Cysticercosis biology.organism_classification medicine.disease medicine.drug_formulation_ingredient Taenia asiatica Human infection Parasitology Research Paper |
| Zdroj: | Food and Waterborne Parasitology Food and Waterborne Parasitology, Vol 25, Iss, Pp e00136-(2021) |
| ISSN: | 2405-6766 |
| Popis: | Taeniosis is a neglected disease, particularly in developing countries, and is caused by infection with the adult tapeworm of either Taenia solium, Taenia saginata, and Taenia asiatica. Of these, T. solium is of primary concern due to the potential for cysticercosis should T. solium eggs be ingested. In Cuba, all cases of taeniosis are assumed to be caused by T. saginata, although some cases of cysticercosis have been documented. It is therefore important to gain further insights regarding the species causing taeniosis in Cuba, especially as diagnostic records indicate an increasing incidence, with the highest number of cases reported in 2020. In this study, we analysed 37 Taenia-positive faecal samples (or proglottids isolated from faecal samples) from the period 2001 until 2020 from all regions of the country. Genomic DNA was extracted from the samples, which had been stored in 10% formalin, using the QIAamp Tissue Kit. Species identification was carried out by duplex real-time PCR targeting the mitochondrial DNA. All cases were found to be T. saginata, and sequence analysis of three isolates confirmed the identification of this species. Our data do not provide any evidence that T. solium currently occurs in Cuba. However, given the relatively low number of samples analysed here, that the parasite may be imported with visitors or travellers who have been in endemic countries, and that taeniosis has relatively mild symptoms and thus infected patients may not seek medical attention, we recommend species determination for all taeniosis cases reported in Cuba. Highlights • The incidence of taeniosis cases in Cuba is rising, with 2020 being a peak year • Although assumed to be Taenia saginata, this has not been confirmed • We investigated 37 samples from 2001 until 2020 that had been stored in formalin • Duplex real-time PCR indicated T. saginata; 3 cases were confirmed by sequencing |
| Databáze: | OpenAIRE |
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