Temporal response of biochemical and biological sensors with bimodal surface adsorption from a finite sample
| Autor: | Miloš Frantlović, Ivana Jokić, Zoran Jakšić, Olga Jakšić, Karumuri Srinivasa Rao, Koushik Guha |
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| Rok vydání: | 2020 |
| Předmět: |
biochemical/biological sensors
Surface (mathematics) Analyte Materials science analyte depletion 02 engineering and technology 01 natural sciences Adsorption 0103 physical sciences Molecule Electrical and Electronic Engineering Reaction chamber bimodal affinity Plasmon 010302 applied physics Mathematical models Mathematical model Technical Paper 021001 nanoscience & nanotechnology Condensed Matter Physics Sample (graphics) Electronic Optical and Magnetic Materials adsorption Hardware and Architecture 0210 nano-technology Biological system |
| Zdroj: | Microsystem Technologies |
| ISSN: | 1432-1858 0946-7076 |
| Popis: | The importance of adsorption-based biochemical/biological sensors in biochemistry and biophysics is paramount. Their temporal response gives information about the presence of a biochemical/biological analyte, its concentration and its interactions with the adsorption sites (which may be an integral part of the surface itself or immobilized functionalizing molecules). Mathematical models of the temporal response taking into account as many relevant effects as possible are essential for obtaining reliable information. We present a novel model taking into account the bimodal affinity of a sensing surface (adsorption occurs on two distinct site types), and the adsorption-caused depletion of the analyte from the sample. We perform qualitative and quantitative analysis of the analyte depletion influence on the bimodal adsorption, and of the influence of the sensing surface inhomogeneity on the sensor temporal response, for different analyte concentrations and different fractions of two types of adsorption sites. Since the presented mathematical model deals with the realistic cases of the sensing surface non-uniformity and the finite amount of analyte present in the sensor reaction chamber, it enables improved accuracy in interpreting the measurement data. Our results are general, i.e. valid for any adsorption sensor (microcantilevers, plasmonics) and for arbitrary sensor dimensions. |
| Databáze: | OpenAIRE |
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