Compositional, ultrastructural and nanotechnological characterization of the SMA strain of Saccharomyces pastorianus: Towards a more complete fermentation yeast cell analysis

Autor: Elizabeth Coetsee, Chantel W. Swart, R. Alex Speers, Suzanne M. Budge, Greg Potter, M.M. Duvenhage, Pieter W. J. van Wyk, Hendrik C. Swart
Rok vydání: 2018
Předmět:
Metabolic Processes
0106 biological sciences
0301 basic medicine
Confocal Microscopy
lcsh:Medicine
Yeast and Fungal Models
Biochemistry
01 natural sciences
Saccharomyces
Mass Spectrometry
Microscopy
Nanotechnology
Electron Microscopy
lcsh:Science
Multidisciplinary
biology
Strain (chemistry)
Chemistry
Eukaryota
Light Microscopy
Saccharomyces pastorianus
Experimental Organism Systems
Saccharomyces Cerevisiae
Engineering and Technology
Scanning Electron Microscopy
Research Article
Saccharomyces cerevisiae
Research and Analysis Methods
03 medical and health sciences
Model Organisms
010608 biotechnology
lcsh:R
Organisms
Fungi
Biology and Life Sciences
biology.organism_classification
Yeast
Metabolism
030104 developmental biology
Fermentation
Microscopy
Electron
Scanning
Biophysics
Ultrastructure
Transmission Electron Microscopy
lcsh:Q
Confocal Laser Microscopy
Zdroj: PLoS ONE, Vol 13, Iss 7, p e0200552 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Nano scanning Auger microscopy (NanoSAM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) have been used in materials science research for some time, but NanoSAM, in particular, has only recently been applied to biological specimens. Here, the first concurrent utilization of NanoSAM, TOF-SIMS and microscopic techniques for the examination of a standard beverage fermentation strain of Saccharomyces pastorianus uncovered the presence of intracellular networks of CO2 in fermenting cells. Respiring cells produced few bubbles and instead had large internal vacuolar structures. Transmission electron microscopy analysis also showed osmiophilic layers at the cell exterior of fermenting cells that became more prevalent with fermentation duration, while osmiophilic layers were largely absent in respiring cells. TOF-SIMS analysis showed a compositional difference at the exterior and interior of SMA cells and between fermenting and respiring cells. Fermenting cells also appeared to have different 3-OH oxylipin profiles compared to respiring cells based upon examination with immunofluorescence microscopy. The results of this work and further study using these materials science techniques will substantially enhance our understanding of the chemical, ultrastructural and metabolic changes that occur in fermentation yeasts.
Databáze: OpenAIRE
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