Formulation and evaluation of targeted nanoparticles for breast cancer theranostic system
| Autor: | Fatemeh Atyabi, Leila Ma’mani, Marzieh Ebrahimi, pegah dadras, Shiva Irani, Zahra Hadavand Mirzaie, Alireza Foroumadi, Rassoul Dinarvand |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Theranostic Nanomedicine
Cell Survival Drug Compounding education Drug Evaluation Preclinical Pharmaceutical Science Silibinin Nanotechnology Breast Neoplasms 02 engineering and technology Polyethylene glycol 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Polylactic Acid-Polyglycolic Acid Copolymer medicine Humans MTT assay Lactic Acid technology industry and agriculture Cancer 021001 nanoscience & nanotechnology medicine.disease PLGA chemistry Targeted drug delivery Docetaxel 030220 oncology & carcinogenesis Cancer research MCF-7 Cells Nanoparticles Female 0210 nano-technology Polyglycolic Acid medicine.drug |
| Zdroj: | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 97 |
| ISSN: | 1879-0720 |
| Popis: | Theranostic polymeric NPs developed for both cancer diagnosis and cancer therapy. This multifunctional polymeric vehicle was prepared by a single emulsion evaporation method, using carboxyl-terminated PLGA. LHRH as a targeting moiety, was conjugated to the surface of polymeric carrier by applying polyethylene glycol. The results indicated that the diameter of NPs was ~185.4±4.6nm as defined by DLS. The entrapment efficacy of docetaxel, silibinin, and SPIONs was 84.6±4.1%, 80.6±2.7%, and 77.9±4.3%, respectively. The NPs showed a triphasic in-vitro drug release pattern. MTT assay was done on two cell lines, MCF-7 and SKOV-3. Enhanced cellular uptake ability of the targeted NPs to MCF-7 was evaluated in-vitro by confocal laser scanning microscopy. The results indicated that compared to non-targeted NPs, the LHRH targeted NPs had significant efficacy at IC50 concentration. The effect of the NPs on VEGF expression in MCF-7 and SKOV-3 cells was investigated by Real-Time PCR method. VEGF mRNA level expression in MCF-7 cell line reduced by 83% in comparison to control cell line. The designed NPs can be used as promising multifunctional platform for detection and targeted drug delivery in breast cancer. |
| Databáze: | OpenAIRE |
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