Effects of methyl methanesulfonate on mouse sperm chromatin structure and testicular cell kinetics
| Autor: | Lorna K. Jost, Donald P. Evenson, Rebecca K. Baer |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
endocrine system Alkylation Epidemiology Health Toxicology and Mutagenesis Pilot Projects Biology Flow cytometry Mice Random Allocation chemistry.chemical_compound Testis medicine Animals Genetics (clinical) Mice Inbred C3H Ploidies Spermatid medicine.diagnostic_test urogenital system Acridine orange Chromosome DNA Flow Cytometry Methyl Methanesulfonate Spermatozoa Sperm Protamine Molecular biology Chromatin Methyl methanesulfonate Mice Inbred C57BL medicine.anatomical_structure chemistry biology.protein Sperm Head |
| Zdroj: | Environmental and Molecular Mutagenesis. 21:144-153 |
| ISSN: | 1098-2280 0893-6692 |
| Popis: | Effects of methyl methanesulfonate (MMS) on mouse testicular cell kinetics and sperm chromatin structure were determined flow cytometrically. Mice were exposed to a single ip injection of saline containing 0 or 150 mg/kg MMS, Relative ratios of 1N, 2N and 4N testicular cells were not affected until 22 days postexposure. Ratios of 1N cell types were altered from 13 to 22 days and were near normal by 25 days. This study revealed an MMS induced alteration of chromatin structure in testicular, elongated spermatids by the sperm chromatin structure assay (SCSA), a flow cytometric measure of the susceptibility of acridine orange stained sperm DNA to denaturation in situ. The SCSA also detected alterations in cauda sperm chromatin structure at 3 days, which was 8 days prior to alterations in sperm head morphology, indicating the increased sensitivity of the SCSA. SCSA data were practically similar whether measuring either fresh or frozen/thawed sperm, or whether measured by two different types of flow cytometers: a) laser driven, orthogonal optical axis; or b) low cost mercury arc lamp system with epiillumination. The data support the model of Sega and Owens [Mutat Res 111:227–244:1983] that MMS alkylates cysteine -SH groups in sperm protamines, thereby destabilizing sperm chromatin structure and leading to broken chromosomes and mutations. © 1993 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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