Assessment of automated high-throughput serological assays for prediction of high-titer SARS-CoV-2 neutralizing antibody
| Autor: | Fabrizio Maggi, Daniele Focosi, Francesco Menichetti, Pietro Villa, Maria Lanza, Michele Lai, Giulia Freer, Mauro Pistello, Irene Bianco, Marco Falcone, A. Mazzoni, Alice Cara, Alfredo Rosellini, Valerio Mainardi, Paola Quaranta, Giovanna Moscato, Paola Mazzetti, Ersilia Lucenteforte |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
IgG
RT-PCR Infectious and parasitic diseases RC109-216 SARS-CoV-2 COVID-19 Neutralizing antibody IgG IgA RT-PCR Neutralization assay EIA CLIA CMIA Article Neutralization Serology law.invention EIA law medicine Microneutralization Assay SARS-CoV-2 COVID-19 Neutralizing antibody IgG IgA RT-PCR Neutralization assay EIA CLIA CMIA Neutralizing antibody biology medicine.diagnostic_test Chemistry SARS-CoV-2 COVID-19 Virology Neutralization assay Titer Immunoassay biology.protein Recombinant DNA CMIA Antibody CLIA IgA |
| Zdroj: | Journal of Clinical Virology Plus Journal of Clinical Virology Plus, Vol 1, Iss 1, Pp 100016-(2021) |
| Popis: | COVID19 convalescent patient plasma units with high titer neutralizing antibody can be used to treat patients with severe disease. Therefore, in order to select suitable donors, neutralizing antibody titer against SARS CoV-2 needs to be determined. Because the neutralization assay is highly demanding from several points of view, a pre-selection of sera would be desirable to minimize the number of sera to be tested. In this study, a total of 140 serum samples that had been titrated for SARS-CoV-2 neutralizing antibody by microneutralization assay were also tested for the presence of anti-SARS-CoV2 antibody using 5 different tests: Architect® immunoassay (Abbott Diagnostics), detecting IgG against the nucleocapsid protein, LIAISON XL® (Diasorin) detecting IgG against a recombinant form of the S1/S2 subunits of the spike protein, VITROS® (Ortho Clinical Diagnostics), detecting IgG against a recombinant form of the spike protein, and ELISA (Euroimmun AG), detecting IgA or IgG against a recombinant form of the S1 subunit. To determine which immunoassay had the highest chance to detect sera with neutralizing antibodies above a certain threshold, we compared the results obtained from the five immunoassays with the titers obtained by microneutralization assay by linear regression analysis and by using receiver operating characteristic curve and Youden's index. Our results indicate that the most suitable method to predict sera with high Nab titer is Euroimmun® IgG, followed closely by Ortho VITROS® Anti-SARS-CoV-2 IgG. |
| Databáze: | OpenAIRE |
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