Assessment of flow cytometry for microbial water quality monitoring in cooling tower water and oxidizing biocide treatment efficiency

Autor: Karim Helmi, Marie-Pierre Jaffrezic, Fabienne David, Patrick Di Martino, Valérie Ingrand
Přispěvatelé: Veolia Recherche et Innovation, Veolia France, Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe), Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT), Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, DI MARTINO, PATRICK
Jazyk: angličtina
Rok vydání: 2018
Předmět:
[SDE] Environmental Sciences
0301 basic medicine
Microbiology (medical)
Biocide
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Sodium Hypochlorite
Legionella
030106 microbiology
Colony Count
Microbial
Statistical difference
010501 environmental sciences
01 natural sciences
Microbiology
Water Purification
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
Adenosine Triphosphate
Water Quality
Oxidizing agent
medicine
Propidium iodide
Cooling tower
Molecular Biology
ComputingMilieux_MISCELLANEOUS
0105 earth and related environmental sciences
Microbial Viability
Chromatography
Bacteria
medicine.diagnostic_test
biology
Water Pollution
Water
Flow Cytometry
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
6. Clean water
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
Disinfection
Kinetics
chemistry
[SDE]Environmental Sciences
Water quality
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Water Microbiology
Disinfectants
Zdroj: Journal of Microbiological Methods
Journal of Microbiological Methods, Elsevier, 2018, 152, pp.201-209
ISSN: 0167-7012
1872-8359
Popis: The control of Legionella proliferation in cooling tower water circuits requires regular monitoring of water contamination and effective disinfection procedures. In this study, flow cytometry was assessed to monitor water contamination and disinfection treatment efficiency on bacterial cells regarding nucleic acid injury (SYBR® Green II), cell integrity (SYBR® Green II and propidium iodide) and metabolism activity (ChemChrome V6). A total of 27 cooling tower water samples were analyzed in order to assess water contamination levels regarding viable populations: standard culture, ATP measurement and flow cytometry methods were compared. Flow cytometry and plate counts methods showed a significant correlation for changes in concentrations despite a 1 to 2-log difference regarding absolute quantification. Concerning intracellular activity, the use of two different flow cytometers (FACSCanto™ II and Accuri™ C6) showed no statistical difference while a difference was observed between flow cytometry and usual methods (culture and ATP measurement). The standard culture and flow cytometry methods were also compared for in vitro bacteria inactivation measurements in the presence of 3 different types of oxidizing biocides commonly used for cooling tower disinfection. Reductions observed ranged between 1 and 2 log depending on (1) the detection method, (2) the bacterial population origin and/or (3) the active biocide molecule used. In conclusion, flow cytometry represents an efficient, accurate and fast approach to monitor water contamination and biocide treatment efficiency in cooling towers.
Databáze: OpenAIRE
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