Prevalence of Sarcocystis spp. in Argentinean cattle
| Autor: | P. Abrahamovich, Magdalena Rambeaud, Gastón Moré, S.B. Jurado, J.C. Marin, María Cecilia Venturini, D. Bacigalupe, L. Venturini |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Veterinary medicine SARCOCYSTIS Sarcocystosis Argentina Cattle Diseases Biology Beef cattle DIAGNOSIS Sarcocystis cruzi Loin PCR-RFLP parasitic diseases Prevalence Animals FOOD SAFETY Kappa value Production area General Veterinary Ciencias Veterinarias Sarcocystis General Medicine biology.organism_classification CIENCIAS AGRÍCOLAS FRESH EXAMINATION Cattle Female Parasitology Restriction fragment length polymorphism Sarcocystis hominis |
| Zdroj: | Veterinary Parasitology. 177:162-165 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2010.11.036 |
| Popis: | Sarcocystis cruzi, S. hirsuta and S. hominis are apicomplexan parasites that affect cattle worldwide with variable prevalence. The aim of the present study was to evaluate the prevalence of Sarcocystis spp. in Argentinean cattle comparing microscopic fresh examination and molecular methods. Blood, myocardium and loin samples were collected in five slaughterhouses from a total of 380 bovines. Origin of animals was representative of the major beef cattle production area of Argentina. Samples were analyzed by fresh microscopical examination, transmission electron microscopy (TEM), IFAT and PCR-RFLP. Thin walled sarcocysts corresponding with S. cruzi were found in 99.5% of heart samples. Sarcocysts were detected in 73.1% of loin samples; 71.5% had S. cruzi cysts and 23.1% had thick walled sarcocysts (S. hirsuta or S. hominis). TEM observation revealed the presence of characteristic S. hominis and S. hirsuta cyst walls in 7 and 1 loin samples respectively. Using IFAT, 379/380 animals had titers 25 or higher, showing a full agreement with fresh examination. Amplification products were detected in 35.5% (135/380) of loin samples; however Sarcocystis species could only be determined by RFLP in 29 samples. Agreement between fresh examination and PCR was low (Kappa value = 0.262). This is the first report of S. hominis and S. hirsuta in Argentina. Further studies are needed to improve the sensitivity of molecular methods for species identification, especially for differentiation of S. cruzi and S. hirsuta from the zoonotic species S. hominis. The results of the present study and others focusing on sensitivity and specificity of Sarcocystis spp. diagnostic methods should contribute to improve food safety. Fil: Moré, Gastón Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina Fil: Abrahamovich, P.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina Fil: Jurado, Susana Beatriz. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina Fil: Bacigalupe, Diana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina Fil: Marin, J.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina Fil: Rambeaud, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina Fil: Venturini, L.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina Fil: Venturini, M.C.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública; Argentina |
| Databáze: | OpenAIRE |
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