Basal and IGF-I-dependent regulation of potassium channels by MAP kinases and PI3-kinase during eccentric cardiac hypertrophy
| Autor: | Aiqiu Zhao, Graham G. Laurence, Zikiar Alvin, Georges E. Haddad, Chuanfu Li, Leyla Teos |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Potassium Channels Time Factors Physiology Mitogen-Activated Protein Kinase 3 Cardiomegaly Biology p38 Mitogen-Activated Protein Kinases Membrane Potentials Rats Sprague-Dawley Phosphatidylinositol 3-Kinases Physiology (medical) Internal medicine medicine Animals Myocyte Myocytes Cardiac Insulin-Like Growth Factor I Phosphorylation Protein Kinase Inhibitors Phosphoinositide-3 Kinase Inhibitors Mitogen-Activated Protein Kinase 1 Membrane potential Kinase Articles Resting potential Potassium channel Rats Disease Models Animal Endocrinology Mitogen-activated protein kinase biology.protein Mitogen-Activated Protein Kinases Signal transduction Cardiology and Cardiovascular Medicine Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 295:H1834-H1845 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.321.2008 |
| Popis: | The potassium channels IK and IK1, responsible for the action potential repolarization and resting potential respectively, are altered during cardiac hypertrophy. The activation of insulin-like growth factor-I (IGF-I) during hypertrophy may affect channel activity. The aim was to examine the modulatory effects of IGF-I on IK and IK1 through mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways during hypertrophy. With the use of specific inhibitors for ERK1/2 (PD98059), p38 MAPK (SB203580) and PI3K/Akt (LY294002), Western blot and whole cell patch-clamp were conducted on sham and aorto-caval shunt-induced hypertrophy adult rat myocytes. Basal activation levels of MAPKs and Akt were increased during hypertrophy. Acute IGF-I (10−8 M) enhanced basal activation levels of these kinases in normal hearts but only those of Akt in hypertrophied ones. IK and IK1 activities were lowered by IGF-I. Inhibition of ERK1/2, p38 MAPK, or Akt reduced basal IK activity by 70, 32, or 50%, respectively, in normal cardiomyocytes vs. 53, 34, or 52% in hypertrophied ones. However, basal activity of IK1 was reduced by 45, 48, or 45% in the former vs. 63, 43, or 24% in the latter. The inhibition of either MAPKs or Akt alleviated IGF-I effects on IK and IK1. We conclude that basal IK and IK1 are positively maintained by steady-state Akt and ERK activities. K+ channels seem to be regulated in a dichotomic manner by acutely stimulated MAPKs and Akt. Eccentric cardiac hypertrophy may be associated with a change in the regulation of the steady-state basal activities of K+ channels towards MAPKs, while that of the acute IGF-I-stimulated ones toward Akt. |
| Databáze: | OpenAIRE |
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