A monoclonal antibody toBabesia divergenswhich inhibits merozoite invasion
| Autor: | J. D. Culverhouse, Elizabeth U. Canning, Caroline M. Winger |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Erythrocytes
medicine.drug_class Babesia Fluorescent Antibody Technique Antigens Protozoan Enzyme-Linked Immunosorbent Assay Monoclonal antibody Epitope Cell Line Epitopes Mice Antigen Western blot Neutralization Tests medicine Animals Direct fluorescent antibody Babesia divergens Gel electrophoresis Mice Inbred BALB C Hybridomas biology medicine.diagnostic_test Antibodies Monoclonal biology.organism_classification Molecular biology Infectious Diseases Antigens Surface biology.protein Electrophoresis Polyacrylamide Gel Animal Science and Zoology Parasitology Antibody |
| Zdroj: | Parasitology. 94:17-27 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | SUMMARYA monoclonal antibody-producing hybridoma cell line has been raised against merozoites ofBabesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes. |
| Databáze: | OpenAIRE |
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