Complement component 3: characterization and association with mastitis resistance in Egyptian water buffalo and cattle
| Autor: | Abd-El-Monsif A. Shawky, Y.A. Ghazi ., Ahmed F.M. Al-Tohamy, Christiane Neuhoff, Nermin El-Halawany, Dessie Salilew-Wondim, Hamdy Abdel-Shafy, Magdy A. Abdel-Latif, Karl Schellander, Lamees Hegazy |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular DNA Complementary Buffaloes Genotype Sequence analysis Protein Conformation animal diseases Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide 03 medical and health sciences Exon Complementary DNA parasitic diseases Genetics medicine Animals Genetic Predisposition to Disease Amino Acid Sequence Mastitis Bovine Alleles Genetic Association Studies Disease Resistance Complement component 3 food and beverages Genetic Variation Hydrogen Bonding Complement C3 Sequence Analysis DNA medicine.disease Complement system Mastitis 030104 developmental biology Phenotype Amino Acid Substitution Mutation Cattle Egypt Female Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of genetics. 96(1) |
| ISSN: | 0973-7731 |
| Popis: | Mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. Complement component C3 plays a major role as a central molecule of the complement cascade involving in killing of microorganisms, either directly or in cooperation with phagocytic cells. C3 cDNA were isolated, from Egyptian buffalo and cattle, sequenced and characterized. The C3 cDNA sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. Buffalo and cattle C3 cDNAs share 99% of sequence identity with each other. The 4986 bp open reading frame in buffalo encodes a putative protein of 1661 amino acids—as in cattle—and includes all the functional domains. Further, analysis of the C3 cDNA sequences detected six novel single-nucleotide polymorphisms (SNPs) in buffalo and three novel SNPs in cattle. The association analysis of the detected SNPs with milk somatic cell score as an indicator of mastitis revealed that the most significant association in buffalo was found in the C >A substitution (ss: 1752816097) in exon 27, whereas in cattle it was in the C >T substitution (ss: 1752816085) in exon 12. Our findings provide preliminary information about the contribution of C3 polymorphisms to mastitis resistance in buffalo and cattle. |
| Databáze: | OpenAIRE |
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