Trabecular Bone Parameters, TIMP-2, MMP-8, MMP-13, VEGF Expression and Immunolocalization in Bone and Cartilage in Newborn Offspring Prenatally Exposed to Fumonisins

Autor: Marta Arczewska, Marcin Bartłomiej Arciszewski, O. M. Brezvyn, Viktor Muzyka, Janine Donaldson, Volodymyr Kushnir, Piotr Dobrowolski, Monika Hułas-Stasiak, Halyna Rudyk, Maria Mielnik-Błaszczak, Ewa Tomaszewska, Izabela Świetlicka, Ihor Kotsyumbas, Siemowit Muszyński
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Vascular Endothelial Growth Factor A
newborn rats
Matrix metalloproteinase
bone
Bone remodeling
Serine
chemistry.chemical_compound
Pregnancy
Growth Plate
Biology (General)
Ceramide synthase
fumonisins (FB)
Spectroscopy
Chemistry
Gene Expression Regulation
Developmental
General Medicine
Computer Science Applications
Vascular endothelial growth factor
medicine.anatomical_structure
Matrix Metalloproteinase 8
Cancellous Bone
matrix metalloproteinase 8 (MMP-8)
Female
tissue inhibitor of metalloproteinases 2 (TIMP-2)
Oxidoreductases
medicine.medical_specialty
Offspring
QH301-705.5
Fumonisins
Catalysis
Article
Inorganic Chemistry
Internal medicine
Matrix Metalloproteinase 13
medicine
Animals
matrix metalloproteinase 13 (MMP-13)
articular cartilage
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Sphingolipids
Tissue Inhibitor of Metalloproteinase-2
Bone Development
Sphingosine
Cartilage
Organic Chemistry
Rats
Endocrinology
Animals
Newborn
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 22
International Journal of Molecular Sciences, Vol 22, Iss 12528, p 12528 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms222212528
Popis: Fumonisins are protein serine/threonine phosphatase inhibitors and potent inhibitors of sphingosine N-acyltransferase (ceramide synthase) disrupting de novo sphingolipid biosynthesis. The experiment was conducted to evaluate the effects of fumonisins (FB) exposure from the 7th day of pregnancy to parturition on offspring bone development. The rats were randomly allocated to either a control group (n = 6), not treated with FBs, or to one of the two groups intoxicated with FBs (either at 60 mg FB/kg b.w. or at 90 mg FB/kg b.w. Numerous negative, offspring sex-dependent effects of maternal FB exposure were observed with regards to the histomorphometry of trabecular bone. These effects were due to FB-inducted alterations in bone metabolism, as indicated by changes in the expression of selected proteins involved in bone development: tissue inhibitor of metalloproteinases 2 (TIMP-2), matrix metalloproteinase 8 (MMP-8), matrix metalloproteinase 13 (MMP-13), and vascular endothelial growth factor (VEGF). The immunolocalization of MMPs and TIMP-2 was performed in trabecular and compact bone, as well as articular and growth plate cartilages. Based on the results, it can be concluded that the exposure of pregnant dams to FB negatively affected the expression of certain proteins responsible for bone matrix degradation in newborns prenatally exposed to FB in a dose- and sex-dependent manner.
Databáze: OpenAIRE
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